Quantitative proteomics reveals Shexiang Baoxin Pill exerts cardioprotective effects by preserving energy metabolism in a rat model of myocardial infarction
文献类型：期刊论文
| 作者 | Yu, Feng2; Yu, Yue1,3,5; Tian, Saisai4; Zhou, Yanting1,3; Chen, Xiangling1,3,5; Ye, Ji4; Liu, Qian1,3,5; Xu, Xike4; Zhou, Hu1,3,5,6 ; Zhang, Weidong2,4
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
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| 出版日期 | 2021-02-10 |
| 卷号 | 266页码:10 |
| ISSN号 | 0378-8741 |
| 关键词 | Shexiang baoxin pill Cardiovascular disease LC-MS/MS Quantitative proteomics Energy metabolism |
| DOI | 10.1016/j.jep.2020.113460 |
| 通讯作者 | Zhou, Hu(zhouhu@simm.ac.cn) ; Zhang, Weidong(wdzhangy@hotmail.com) |
| 英文摘要 | Ethnopharmacological relevance: Shexiang Baoxin Pill (SBP) is a composite formula of traditional Chinese medicine used to treat cardiovascular disease (CVD) in the clinic. However, the mechanism of its therapeutic effect on CVD has not been clearly elucidated yet. Aim of the study: The aim of this study was to investigate the potential cardioprotective mechanism of SBP in the treatment of myocardial infarction (MI) model rats by applying proteomic approach. Materials and methods: The rat model of MI was generated by ligating the left anterior descending coronary artery. Eighteen rats were randomly divided into three groups (n = 6 each): the MI group, MI group treated with SBP (SBP), and sham-operated group (SOG). Cardiac function in the experimental groups was assessed by echocardiography analyses after 15 days of treatment. A label-free quantitative proteomic approach was utilized to investigate the whole proteomes of heart tissues from the groups above on the day of the operation (Day 0) and 15 days later (Day 15). The differentially expressed proteins were subsequently analyzed with bioinformatic methods. Additionally, the expression levels of two promising proteins were validated by Western blotting. Results: The echocardiography analyses showed that SBP treatment significantly preserved the cardiac function of MI rats. Additionally, quantitative proteomics identified 389 differentially expressed proteins, and 15 proteins were considered as logical candidates for explaining the cardioprotective effect of SBP. Bioinformatic analysis of these differentially expressed proteins revealed that the proteins involved in cellular mitochondrial energy metabolism processes, such as fatty acid beta-oxidation and aerobic respiration, were significantly regulated under SBP treatment, of which fatty acid-binding protein 3 (FABP3) and myoglobin (MB) were significantly downregulated in the MI model group compared with the SOG group and returned to the basal level with SBP treatment, confirmed by Western blotting. Conclusions: The results of our study suggest that the cardioprotective effects of SBP are achieved through the preservation of energy metabolism in the heart tissue of MI rats. |
| 资助项目 | National Key Research and Development Program of China[2019YFC1711000] ; National Key Research and Development Program of China[2017YFC1700200] ; Professor of Chang Jiang Scholars Program, National Natural Science Foundation of China[81520108030] ; Professor of Chang Jiang Scholars Program, National Natural Science Foundation of China[21472238] ; Professor of Chang Jiang Scholars Program, National Natural Science Foundation of China[82073981] ; Professor of Chang Jiang Scholars Program, National Natural Science Foundation of China[82004003] ; Shanghai Engineering Research Center for the Preparation of Bioactive Natural Products[16DZ2280200] ; Scientific Foundation of Shanghai China[13401900103] ; Scientific Foundation of Shanghai China[13401900101] ; Shanghai Sailing Program[20YF1459000] ; National Science and Technology Major Project of China[2019ZX09201005-007-003] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| 出版者 | ELSEVIER IRELAND LTD |
| WOS记录号 | WOS:000598059400001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/296369]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhou, Hu; Zhang, Weidong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Medica, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Integrat Med Res, 1200 Cailun Rd, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 4.Second Mil Med Univ, Sch Pharm, Shanghai 200433, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Municipal Educ Comm, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Feng,Yu, Yue,Tian, Saisai,et al. Quantitative proteomics reveals Shexiang Baoxin Pill exerts cardioprotective effects by preserving energy metabolism in a rat model of myocardial infarction[J]. JOURNAL OF ETHNOPHARMACOLOGY,2021,266:10. |
| APA | Yu, Feng.,Yu, Yue.,Tian, Saisai.,Zhou, Yanting.,Chen, Xiangling.,...&Zhang, Weidong.(2021).Quantitative proteomics reveals Shexiang Baoxin Pill exerts cardioprotective effects by preserving energy metabolism in a rat model of myocardial infarction.JOURNAL OF ETHNOPHARMACOLOGY,266,10. |
| MLA | Yu, Feng,et al."Quantitative proteomics reveals Shexiang Baoxin Pill exerts cardioprotective effects by preserving energy metabolism in a rat model of myocardial infarction".JOURNAL OF ETHNOPHARMACOLOGY 266(2021):10. |
入库方式： OAI收割
来源：上海药物研究所
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