Protective Effects of 28-O-Caffeoyl Betulin (B-CA) on the Cerebral Cortex of Ischemic Rats Revealed by a NMR-Based Metabolomics Analysis
文献类型：期刊论文
| 作者 | Liu, Xia2 ; Ruan, Zhi3; Shao, Xing-cheng1,4; Feng, Hong-xuan3; Wu, Lei3; Wang, Wei3; Wang, Hong-min4; Mu, Hong-yan1,4; Zhang, Ru-jun4; Zhao, Wei-min4
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| 刊名 | NEUROCHEMICAL RESEARCH
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| 出版日期 | 2021-01-03 |
| 页码 | 13 |
| 关键词 | Cerebral ischemia– reperfusion 28-O-caffeoyl betulin Neuroprotection Metabolomics NMR |
| ISSN号 | 0364-3190 |
| DOI | 10.1007/s11064-020-03202-z |
| 通讯作者 | Zhao, Wei-min(wmzhao@simm.ac.cn) ; Zhang, Hai-yan(hzhang@simm.ac.cn) ; Zhang, Nai-xia(nxzhang@simm.ac.cn) |
| 英文摘要 | 28-O-caffeoyl betulin (B-CA) has been demonstrated to reduce the cerebral infarct volume caused by transient middle cerebral artery occlusion (MCAO) injury. B-CA is a novel derivative of naturally occurring caffeoyl triterpene with little information associated with its pharmacological target(s). To date no data is available regarding the effect of B-CA on brain metabolism. In the present study, a H-1-NMR-based metabolomics approach was applied to investigate the therapeutic effects of B-CA on brain metabolism following MCAO in rats. Global metabolic profiles of the cortex in acute period (9 h after focal ischemia onset) after MCAO were compared between the groups (sham; MCAO + vehicle; MCAO + B-CA). MCAO induced several changes in the ipsilateral cortex of ischemic rats, which consequently led to the neuronal damage featured with the downregulation of NAA, including energy metabolism dysfunctions, oxidative stress, and neurotransmitter metabolism. Treatment with B-CA showed statistically significant rescue effects on the ischemic cortex of MCAO rats. Specifically, treatment with B-CA ameliorated the energy metabolism dysfunctions (back-regulating the levels of succinate, lactate, BCAAs, and carnitine), oxidative stress (upregulating the level of glutathione), and neurotransmitter metabolism disturbances (back-regulating the levels of gamma-aminobutyric acid and acetylcholine) associated with the progression of ischemic stroke. With the administration of B-CA, the levels of three phospholipid related metabolites (O-phosphocholine, O-phosphoethanolamine, sn-glycero-3-phosphocholine) and NAA improved significantly. Overall, our findings suggest that treatment with B-CA may provide neuroprotection by augmenting the metabolic changes observed in the cortex following MCAO in rats. |
| WOS关键词 | ARTERY OCCLUSION ; N-ACETYLASPARTATE ; AMINO-ACID ; STROKE ; METABOLISM ; TAURINE ; ACETYLCHOLINE ; TRANSIENT ; METABONOMICS ; REPERFUSION |
| 资助项目 | National Natural Science Foundation of China[21877119] ; National Natural Science Foundation of China[81872859] ; National Natural Science Foundation of China[21778061] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program of China[2018ZX09711002] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program of China[2018ZX09711002-002-014] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000604515400001 |
| 出版者 | SPRINGER/PLENUM PUBLISHERS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/296390]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhao, Wei-min; Zhang, Hai-yan; Zhang, Nai-xia |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, CAS Key Lab Receptor Res, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Nat Prod Chem, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xia,Ruan, Zhi,Shao, Xing-cheng,et al. Protective Effects of 28-O-Caffeoyl Betulin (B-CA) on the Cerebral Cortex of Ischemic Rats Revealed by a NMR-Based Metabolomics Analysis[J]. NEUROCHEMICAL RESEARCH,2021:13. |
| APA | Liu, Xia.,Ruan, Zhi.,Shao, Xing-cheng.,Feng, Hong-xuan.,Wu, Lei.,...&Zhang, Nai-xia.(2021).Protective Effects of 28-O-Caffeoyl Betulin (B-CA) on the Cerebral Cortex of Ischemic Rats Revealed by a NMR-Based Metabolomics Analysis.NEUROCHEMICAL RESEARCH,13. |
| MLA | Liu, Xia,et al."Protective Effects of 28-O-Caffeoyl Betulin (B-CA) on the Cerebral Cortex of Ischemic Rats Revealed by a NMR-Based Metabolomics Analysis".NEUROCHEMICAL RESEARCH (2021):13. |
入库方式： OAI收割
来源：上海药物研究所
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