Three dimensional drift control at nano-scale in single molecule localization microscopy
文献类型：期刊论文
| 作者 | Fan, Xiaoming1,2,3; Gensch, Thomas3; Buldt, Georg1; Zhang, Yuanheng2,4; Musha, Zulipali2,4; Zhang, Wenyuan2,4; Roncarati, Renza2; Huang, Ruimin2,4
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| 刊名 | OPTICS EXPRESS
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| 出版日期 | 2020-10-26 |
| 卷号 | 28期号:22页码:32750-32763 |
| ISSN号 | 1094-4087 |
| DOI | 10.1364/OE.404123 |
| 通讯作者 | Huang, Ruimin(rmhuang@simm.ac.cn) |
| 英文摘要 | Super-resolution imaging based on single molecule localization of cellular structures on nanometer scale requires to record a series of wide-field or TIRF images resulting in a considerable recording time (typically of minutes). Therefore, sample drift becomes a critical problem and will lower the imaging precision. Herein we utilized morphological features of the specimen (mammalian cells) itself as reference markers replacing the traditionally used markers (e.g., artificial fiduciary markers, fluorescent beads, or metal nanoparticles) for sample drift compensation. We achieved sub-nanometer localization precision <1.0 nm in lateral direction and <6.0 nm in axial direction, which is well comparable with the precision achieved with the established methods using artificial position markers added to the specimen. Our method does not require complex hardware setup, extra labelling or markers, and has the additional advantage of the absence of photobleaching, which caused precision decrease during the course of superresolution measurement. The achieved improvement of quality and resolution in reconstructed super-resolution images by application of our drift-correction method is demonstrated by single molecule localization-based super-resolution imaging of F-actin in fixed A549 cells. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
| WOS关键词 | OPTICAL RECONSTRUCTION MICROSCOPY |
| 资助项目 | National Natural Science Foundation of China[81771890] ; National Natural Science Foundation of China[91859106] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program, China[2018ZX09711002-010-001] ; One Hundred Talent Program of Chinese Academy of Sciences ; Instrument Function Development Project of Chinese Academy of Sciences |
| WOS研究方向 | Optics |
| 语种 | 英语 |
| 出版者 | OPTICAL SOC AMER |
| WOS记录号 | WOS:000582499400039 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/296424]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Huang, Ruimin |
| 作者单位 | 1.Forschungszentrum Julich, Inst Complex Syst ICS 5, Mol Biophys, Wilhelm Jonen Str, D-52428 Julich, Germany 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Forschungszentrum Julich, Inst Complex Syst ICS 4, Cellular Biophys, Wilhelm Jonen Str, D-52428 Julich, Germany 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Xiaoming,Gensch, Thomas,Buldt, Georg,et al. Three dimensional drift control at nano-scale in single molecule localization microscopy[J]. OPTICS EXPRESS,2020,28(22):32750-32763. |
| APA | Fan, Xiaoming.,Gensch, Thomas.,Buldt, Georg.,Zhang, Yuanheng.,Musha, Zulipali.,...&Huang, Ruimin.(2020).Three dimensional drift control at nano-scale in single molecule localization microscopy.OPTICS EXPRESS,28(22),32750-32763. |
| MLA | Fan, Xiaoming,et al."Three dimensional drift control at nano-scale in single molecule localization microscopy".OPTICS EXPRESS 28.22(2020):32750-32763. |
入库方式： OAI收割
来源：上海药物研究所
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