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Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers

文献类型：期刊论文

作者Chai, Xianzhi2,3; Han, Hai-Hao2,3,4; Sedgwick, Adam C.5; Li, Na1; Zang, Yi4; James, Tony D.6; Zhang, Junji2,3; Hu, Xi-Le2,3; Yu, Yang1; Li, Yao1
刊名JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
出版日期2020-10-21
卷号142期号:42页码:18005-18013
ISSN号0002-7863
DOI10.1021/jacs.0c05379
通讯作者Zhang, Junji(zhangjunji@ecust.edu.cn) ; Li, Jia(jli@simm.ac.cn) ; He, Xiao-Peng(xphe@ecust.edu.cn)
英文摘要Here, we report a beta-galactosidase (beta-Gal)-responsive photochromic fluorescent probe, NpG, that was designed to prebind to human serum albumin (HSA) to form the probe/protein hybrid, NpG@HSA. The formation of NpG@HSA led to an increase in fluorescence emission (520 nm) corresponding to the binding of the fluorescent naphthalimide unit with HSA. In addition, this enabled visualization of the spiropyran fluorescence emission in aqueous media. Our probe/protein hybrid approach afforded a unique imaging platform with enhanced cell permeability and solubility that was capable of visualizing the cellular uptake of NpG@HSA before its activation by beta-Gal. The beta-Gal-mediated cleavage of the galactose unit within the NpG@HSA hybrid resulted in the formation of NpM@HSA and an increase in red fluorescence emission (620 nm). The resultant merocyanine unit was then able to undergo photoisomerization (merocyanine <-> spiropyran) to facilitate STORM (i.e., stochastic optical reconstruction microscopy) imaging with minimal phototoxicity and excellent photostability/reversibility. Using STORM, NpG@HSA was able to determine the subcellular distribution of beta-Gal activity between cell lines with nanoscale precision. We believe that this system represents a versatile imaging platform for the design of photochromic fluorescent probes suitable for illuminating the precise location of disease-specific biomarkers in various cellular processes.
WOS关键词INTRAMOLECULAR SPIROCYCLIZATION ; POSTTRANSLATIONAL MODIFICATIONS ; IONIZING-RADIATION ; BETA-GALACTOSIDASE ; FLUOROPHORES ; SENESCENCE ; CELLS ; NANOPARTICLES
资助项目NSFC[21788102] ; NSFC[91853201] ; NSFC[21878086] ; NSFC[21722801] ; Shanghai Municipal Science and Technology Major Project[2018SHZDZX03] ; National Key Sci-Tech Special Projects of Infection Diseases of China[2018ZX10732202] ; International Cooperation Program of the Shanghai Science and Technology Committee[17520750100] ; Shanghai Rising-Star Program[19QA1402500] ; Royal Society
WOS研究方向Chemistry
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000580559000024
源URL[http://119.78.100.183/handle/2S10ELR8/296465]  
专题新药研究国家重点实验室
通讯作者Zhang, Junji; Li, Jia; He, Xiao-Peng
作者单位1.Chinese Acad Sci, Natl Ctr Prot Sci Shanghai, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China
2.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China
3.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Joint Int Res Lab Precis Chem, Shanghai 200237, Peoples R China
4.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, State Key Lab Drug Res, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China
5.Univ Texas Austin, Dept Chem, Austin, TX 78712 USA
6.Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England
推荐引用方式
GB/T 7714		 Chai, Xianzhi,Han, Hai-Hao,Sedgwick, Adam C.,et al. Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2020,142(42):18005-18013.
APA Chai, Xianzhi.,Han, Hai-Hao.,Sedgwick, Adam C..,Li, Na.,Zang, Yi.,...&Tian, He.(2020).Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,142(42),18005-18013.
MLA Chai, Xianzhi,et al."Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 142.42(2020):18005-18013.

入库方式： OAI收割

来源：上海药物研究所

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