Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa
文献类型：期刊论文
| 作者 | Yao Shuai1; Zhang Jian-Qing1; Hou Jin-Jun1 ; Hu Xiao-Su1; Wang Ling1; Da Juan1; Rao Wei2; Wang Dan-Dan2; Huang Yong1; Wu Wan-Ying1
|
| 刊名 | CHINESE JOURNAL OF NATURAL MEDICINES
 |
| 出版日期 | 2021-04-01 |
| 卷号 | 19期号:4页码:295-304 |
| 关键词 | High performance thin-layer chromatography Mass spectrometry QDa Ganoderma Authentication |
| ISSN号 | 2095-6975 |
| DOI | 10.1016/S1875-5364(21)60030-4 |
| 通讯作者 | Wu Wan-Ying(wanyingwu@simm.ac.cn) ; Guo De-An(daguo@simm.ac.cn) |
| 英文摘要 | In this study, a high performance thin-layer chromatography/single quadrupole mass spectrometry QDa (HPTLC-QDa) method for robust authentication of Ganoderma lucidum, a popular and valuable herbal medicine, has been developed. This method is simple and practical, which allows direct generation of characteristic mass spectra from the HPTLC plates automatically with the application of in situ solvent desorption interface. The HPTLC silica gel plates were developed with toluene-ethyl formate-formic acid (5 : 5 : 0.2, V/V) and all bands were transferred to QDa system directly in situ using 80% methanol with 0.1% formic acid as desorption solvent. The acquired HPTLC-QDa spectra showed that luminous yellow band b3, containing ganoderic acid B/G/H and ganodeneric acid B, the major active components of Ganoderma, could be found only in G. lucidum and G. lucidum (Antler-shaped), but not in G. sinense and G. applanatum. Moreover, bands b13 and b14 with m/z 475/477 and m/z 475/491/495, respectively, could be detected in G. lucidum (Antler-shaped), but not in G. lucidum, thus allowing simple and robust authentication of G. lucidum with confused species. This method is proved to be simple, practical and reproducible, which can be extended to analyze other herbal medicines. |
| WOS关键词 | CHINESE HERBAL MEDICINES ; GAS-CHROMATOGRAPHY ; QUALITY-CONTROL ; IDENTIFICATION ; LUCIDUM ; TRITERPENOIDS ; LINGZHI ; FINGERPRINTS ; MORPHOLOGY ; RADIX |
| 资助项目 | National Key R&D Program of China[2019YFC1711000] ; National Key R&D Program of China[2018YFC1707900] ; National Natural Science Foundation of China[8200140730] ; Qi-Huang Scholar of National Traditional Chinese Medicine Leading Talents Support Program (2018) |
| WOS研究方向 | Integrative & Complementary Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000641160300005 |
| 出版者 | CHINESE JOURNAL NATURAL MEDICINES |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/296548]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wu Wan-Ying; Guo De-An |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Waters Technol Shanghai Co Ltd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao Shuai,Zhang Jian-Qing,Hou Jin-Jun,et al. Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2021,19(4):295-304. |
| APA | Yao Shuai.,Zhang Jian-Qing.,Hou Jin-Jun.,Hu Xiao-Su.,Wang Ling.,...&Guo De-An.(2021).Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa.CHINESE JOURNAL OF NATURAL MEDICINES,19(4),295-304. |
| MLA | Yao Shuai,et al."Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa".CHINESE JOURNAL OF NATURAL MEDICINES 19.4(2021):295-304. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。