A sustainable pH shift control strategy for efficient production of beta-poly(L-malic acid) with CaCO3 addition by Aureobasidium pullulans ipe-1
文献类型：期刊论文
| 作者 | Cao, Weifeng1,4; Cao, Weilei1,4; Shen, Fei1,4; Luo, Jianquan1,4; Yin, Junxiang3; Qiao, Changsheng2; Wan, Yinhua1,4 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2020-10-01 |
| 卷号 | 104期号:20页码:8691-8703 |
| ISSN号 | 0175-7598 |
| 关键词 | beta-poly(L-malic acid) Aureobasidium pullulans Neutralizer Metabolic pathway |
| DOI | 10.1007/s00253-020-10815-5 |
| 英文摘要 | beta-poly(L-malic acid) (PMLA) has attracted industrial interest for its potential applications in medicine and other industries. For a sustainable PMLA production, it requires replacing/reducing the CaCO3 usage, since the residual CaCO3 impeded the cells' utilization, and a large amount of commercially useless gypsum was accumulated. In this study, it was found that more glucose was converted into CO(2)using soluble alkalis compared with CaCO3 usage. Moreover, since the high ion strength and respiration effect of soluble alkalis also inhibited PMLA production, they could not effectively replace CaCO3. Furthermore, comparing the fermentations with different neutralizers (soluble alkali vs. CaCO3), it was found that the differential genes are mainly involved in the pathway of starch and sucrose metabolism, pentose and glucuronate interconversions, histidine metabolism, ascorbate and aldarate metabolism, and phagosome. In detail, in the case with CaCO3, 562 genes were downregulated and 262 genes were upregulated, and especially, those genes involved in energy production and conversion were downregulated by 26.7%. Therefore, the irreplaceability of CaCO3 was caused by its effect on the PMLA metabolic pathway rather than its usage as neutralizer. Finally, a combined pH shift control strategy with CaCO3 addition was developed. After the fermentation, 64.8 g/L PMLA and 38.9 g/L biomass were obtained with undetectable CaCO3 and less CO2 emission. |
| WOS关键词 | EXPANDED-BED ADSORPTION ; GAMMA-AMINOBUTYRIC-ACID ; ION-EXCHANGE-RESIN ; MALIC-ACID ; POLY(BETA-MALIC ACID) ; POLYMALIC ACID ; SUCCINIC ACID ; FERMENTATION ; SEPARATION ; BROTH |
| 资助项目 | Beijing Natural Science Foundation, China[5182025] ; National Natural Science Foundation of China, China[21406240] ; National High Technology Research and Development Program of China[2015AA021002] ; National High Technology Research and Development Program of China[2014AA021005] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000567768900004 |
| 资助机构 | Beijing Natural Science Foundation, China ; National Natural Science Foundation of China, China ; National High Technology Research and Development Program of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/42006]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Cao, Weifeng; Wan, Yinhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Bioengn, Tianjin 300457, Peoples R China 3.China Natl Ctr Biotechnol Dev, Beijing 100036, Peoples R China 4.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Weifeng,Cao, Weilei,Shen, Fei,et al. A sustainable pH shift control strategy for efficient production of beta-poly(L-malic acid) with CaCO3 addition by Aureobasidium pullulans ipe-1[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020,104(20):8691-8703. |
| APA | Cao, Weifeng.,Cao, Weilei.,Shen, Fei.,Luo, Jianquan.,Yin, Junxiang.,...&Wan, Yinhua.(2020).A sustainable pH shift control strategy for efficient production of beta-poly(L-malic acid) with CaCO3 addition by Aureobasidium pullulans ipe-1.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,104(20),8691-8703. |
| MLA | Cao, Weifeng,et al."A sustainable pH shift control strategy for efficient production of beta-poly(L-malic acid) with CaCO3 addition by Aureobasidium pullulans ipe-1".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 104.20(2020):8691-8703. |
入库方式： OAI收割
来源：过程工程研究所
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