中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights

文献类型:期刊论文

作者Li, Ke2,3,4; Li, Shuying1,3,4; Du, Yuguang2; Qin, Xuemei3,4
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2020-09-01
卷号1152页码:13
关键词Astragalus polysaccharides for injection Innate and adaptive immunity Proliferation Phagocytic Structural analysis
ISSN号1570-0232
DOI10.1016/j.jchromb.2020.122255
英文摘要As a special traditional Chinese medicine, Astragalus polysaccharides for injection (APS, batch no. Zhunzi Z20040086) includes complex polysaccharide macromolecules that may increase the risk upon application. Although fingerprints for quality control are available, the specific active ingredients are unclear. Identifying the active components is the key to reduce the risk of adverse reactions of the drug. In this work, APS was mainly separated into two components, namely, macromolecular component (APS-I) and small molecular components (APS-II). The molecular weight measurement revealed that the average molecular weight of APS-I exceeded 500 kDa, and that of APS-II was 10 kDa. Monosaccharide-composition analysis revealed that APS-I consisted of glucose, galactose, arabinose, rhamnose, and galacturonic acid, with a ratio of approximately 1.5:1:5.4:0.08:0.1. Meanwhile, APS-II consisted of glucose, galactose, arabinose, rhamnose, and galacturonic acid, with a molar ratio of 9:1:1.4:0.04:0.001. Methylation, FT-IR, and NMR analysis indicated that the APS-I monosaccharide residue was linked as follows: D-Glcp-(1 ->, -> 4)-D-Glcp-(1 ->, -> 2)-L-Rhap-(1 ->, D-Araf-(1 ->, -> 5)-D-Araf-(1 ->, -> 2,5)-D-Araf-(1 ->, -> 4)-D-Galp-(1 -> . Meanwhile, the APS-II monosaccharide residue was connected as follows: alpha-D-Glcp-(1 ->,-> 4)-alpha-D-Glcp-(1 ->, -> 6)-alpha-D-Glcp-(1 ->, -> 4,6)-alpha-D-Glcp-(1 ->, -> 3,4,6)-alpha-D-Glcp-(1 ->, -> 2)-alpha-L-Rhap-(1 ->, alpha-D-Araf-(1 ->, -> 5)-alpha-D-Araf-(1 ->, -> 4)-beta-D-Galp-(1 -> . Screening experiments on their in vitro immunological activity showed that APS-II had stronger effect on innate and adaptive immunities than APS-I. In vivo animal experiments showed that APS-II can increase the leukocyte level of cyclophosphamide immunosuppressed mice and improve their immunomodulatory ability. Therefore, APS-II is the main active ingredient of APS and is expected to become a new generation of APS products.
WOS关键词IMMUNOMODULATORY ACTIVITY ; ANTIOXIDANT ACTIVITIES
资助项目National Natural Science Foundation of China[81872962] ; National Key R&D Program of China[2019YFC1710800] ; China Postdoctoral Science Foundation Project[2019M650851] ; Science and Technology Research Project of Shanxi Province (China)[2014ZD0401] ; Key Projects of Key Research and Development Plan in Shanxi (China)[201603D311101] ; Shanxi Province Technology Innovation Project of Excellent Talent (China)[201605D211030] ; Shanxi Province Technology Innovation Project of Excellent Talent (China)[201705D211020]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000566904300023
出版者ELSEVIER
资助机构National Natural Science Foundation of China ; National Key R&D Program of China ; China Postdoctoral Science Foundation Project ; Science and Technology Research Project of Shanxi Province (China) ; Key Projects of Key Research and Development Plan in Shanxi (China) ; Shanxi Province Technology Innovation Project of Excellent Talent (China)
源URL[http://ir.ipe.ac.cn/handle/122111/42025]  
专题中国科学院过程工程研究所
通讯作者Du, Yuguang; Qin, Xuemei
作者单位1.Shanxi Univ, Coll Chem & Chem Engn, Taiyuan 030006, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
3.Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan 030006, Peoples R China
4.Shanxi Univ, Engn Minist, Key Lab Chem Biol & Mol, Taiyuan 030006, Peoples R China
推荐引用方式
GB/T 7714
Li, Ke,Li, Shuying,Du, Yuguang,et al. Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2020,1152:13.
APA Li, Ke,Li, Shuying,Du, Yuguang,&Qin, Xuemei.(2020).Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1152,13.
MLA Li, Ke,et al."Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1152(2020):13.

入库方式: OAI收割

来源:过程工程研究所

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