Green preparation of hydrogel particles-in-emulsions for simultaneous enhancement of humoral and cell-mediated immunity
文献类型：期刊论文
| 作者 | Zou, Yongjuan2,3; Li, Shuai2,3; Ngai, To4; Zhang, Songping2,3; Ma, Guanghui1,2,3,5; Wu, Jie1,2,3,5 |
| 刊名 | ENGINEERING IN LIFE SCIENCES
 |
| 出版日期 | 2020-09-21 |
| 页码 | 11 |
| 关键词 | cellular immune responses green preparation humoral immunity oil-in-water emulsions quaternized chitosan particles |
| ISSN号 | 1618-0240 |
| DOI | 10.1002/elsc.202000011 |
| 英文摘要 | Emulsions are one of the most often used vaccine adjuvant formulations. Although they promote high humoral immunity, the induced cellular immunity is often poor, which restrict their application. To enhance the cellular immunity, some researchers have prepared mixed formulations by adding particles into the aqueous phase of emulsions. However, the particle preparation process usually involves the addition and removal of organic reagents, which is environmentally unfriendly and cumbersome. Moreover, the obtained vaccine adjuvant only induces limited cell-mediated immunity and humoral immunity compared with emulsion-adjuvanted vaccines. Herein, we developed a green and simple method for fabricating a novel nanoparticles-in-emulsions (NPE) formulation. Firstly, a temperature-sensitive hydrogel was used to prepare particles by self-solidification without additional crosslinking reagents. Secondly, the white oil was used as organic phase to avoid the particle washing procedures and organic solvent residues. Moreover, the effect of NPE as vaccine adjuvant was evaluated by using two veterinary vaccines as model antigens. NPE showed advantages than the conventional vaccine formulations in inducing both humoral and cellular immunity. This work provides a facile and broadly applicable approach for preparing nanoparticles-in-emulsions formulation, and presents an effective adjuvant for enhancing immunity against infectious diseases. |
| WOS关键词 | MOUTH-DISEASE VIRUS ; MEMBRANE EMULSIFICATION ; CHITOSAN MICROSPHERES ; ADJUVANT COMBINATION ; FMDV VACCINE ; RESPONSES ; DELIVERY ; MICROPARTICLES ; VETERINARY ; RELEASE |
| 资助项目 | National Key R&D Program of China[2018YFC0311303] ; National Natural Science Foundation of China[21576268] ; National Natural Science Foundation of China[21776287] ; National Natural Science Foundation of China[21821005] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-218] ; International Cooperation Project of the Chinese Academy of Sciences[122111KYSB20180021] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000571690000001 |
| 出版者 | WILEY |
| 资助机构 | National Key R&D Program of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; International Cooperation Project of the Chinese Academy of Sciences |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/42212]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Wu, Jie |
| 作者单位 | 1.Jiangsu Natl Synerget Innovat Ctr Adv Mat, Nanjing, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, North 2nd St, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Sch Chem Engn, Beijing, Peoples R China 4.Chinese Univ Hong Kong, Dept Chem, Shatin, Hong Kong, Peoples R China 5.Chinese Acad Sci, Inst Proc Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Yongjuan,Li, Shuai,Ngai, To,et al. Green preparation of hydrogel particles-in-emulsions for simultaneous enhancement of humoral and cell-mediated immunity[J]. ENGINEERING IN LIFE SCIENCES,2020:11. |
| APA | Zou, Yongjuan,Li, Shuai,Ngai, To,Zhang, Songping,Ma, Guanghui,&Wu, Jie.(2020).Green preparation of hydrogel particles-in-emulsions for simultaneous enhancement of humoral and cell-mediated immunity.ENGINEERING IN LIFE SCIENCES,11. |
| MLA | Zou, Yongjuan,et al."Green preparation of hydrogel particles-in-emulsions for simultaneous enhancement of humoral and cell-mediated immunity".ENGINEERING IN LIFE SCIENCES (2020):11. |
入库方式： OAI收割
来源：过程工程研究所
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