Manipulation of pore structure during manufacture of agarose microspheres for bioseparation
文献类型：期刊论文
| 作者 | Zhao, Lan2; Huang, Yongdong2; Zhu, Kai2; Miao, Zhuang2; Zhao, Jiazhang2; Che, Xiang Jing2,3; Hao, Dongxia2; Zhang, Rongyue4; Ma, Guanghui1,2 |
| 刊名 | ENGINEERING IN LIFE SCIENCES
 |
| 出版日期 | 2020-09-23 |
| 页码 | 10 |
| 关键词 | agarose microspheres agarose types crosslinking degree gel filtration chromatography pore structure |
| ISSN号 | 1618-0240 |
| DOI | 10.1002/elsc.202000023 |
| 英文摘要 | Agarose microspheres with a controllable pore structure were manufactured by varying agarose types and crosslinking degrees. Various agarose could tailor the gel formation of microspheres matrix and thus affect the final pore structures. Small pores in microspheres could be fabricated by agarose with a higher molecular weight, which was demonstrated by the packed column with lower distribution coefficient (K-av) values measured by gel filtration chromatography. Further, higherK(av)values also demonstrated that more and larger pores were formed with increasing the crosslinking degree of agarose microspheres. Either using agarose with a high molecular weight or increasing the crosslinking degree would finally lead to the enhancement of the flow rate during flow performance of packed column as necessary for improving separation efficiency. This provides a foundation for high-resolution chromatography with a controllable separation range as beneficial for downstream process. |
| WOS关键词 | GEL-BOUND ENZYMES ; CHROMATOGRAPHY ; EMULSIFICATION ; ADSORPTION ; ELECTROPHORESIS ; DERIVATIVES ; DIFFUSION ; UNIFORM ; BEADS ; SIZE |
| 资助项目 | National Key Research and Development Program of China[2018YFC0311101] ; Beijing Municipal Natural Science Foundation - Haidian Original Innovation Joint Fund of China[L192017] ; National Natural Science Foundation of China[21676275] ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee[ZZB2019001] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000572075400001 |
| 出版者 | WILEY |
| 资助机构 | National Key Research and Development Program of China ; Beijing Municipal Natural Science Foundation - Haidian Original Innovation Joint Fund of China ; National Natural Science Foundation of China ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/42231]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Huang, Yongdong; Ma, Guanghui |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Yanshan Univ, Coll Environm & Chem Engn, Qinhuangdao, Hebei, Peoples R China 4.Beijing Inst Petrochem Technol, Coll Chem Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Lan,Huang, Yongdong,Zhu, Kai,et al. Manipulation of pore structure during manufacture of agarose microspheres for bioseparation[J]. ENGINEERING IN LIFE SCIENCES,2020:10. |
| APA | Zhao, Lan.,Huang, Yongdong.,Zhu, Kai.,Miao, Zhuang.,Zhao, Jiazhang.,...&Ma, Guanghui.(2020).Manipulation of pore structure during manufacture of agarose microspheres for bioseparation.ENGINEERING IN LIFE SCIENCES,10. |
| MLA | Zhao, Lan,et al."Manipulation of pore structure during manufacture of agarose microspheres for bioseparation".ENGINEERING IN LIFE SCIENCES (2020):10. |
入库方式： OAI收割
来源：过程工程研究所
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