Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology
文献类型：期刊论文
| 作者 | Wu, Fengli1,2; Chen, Wujiu2; Peng, Yanfeng2; Tu, Ran2; Lin, Yuping2; Xing, Jianmin1; Wang, Qinhong2 |
| 刊名 | ACS SYNTHETIC BIOLOGY
 |
| 出版日期 | 2020-09-18 |
| 卷号 | 9期号:9页码:2399-2409 |
| 关键词 | Vibrio natriegens synthetic regulatory parts combinatorial modulation glucose transport synthetic biology |
| ISSN号 | 2161-5063 |
| DOI | 10.1021/acssynbio.0c00158 |
| 英文摘要 | The fast-growing Vibrio natriegens is an attractive robust chassis for diverse synthetic biology applications. However, V. natriegens lacks the suitable constitutive regulatory parts for precisely tuning the gene expression and, thus, recapitulating physiologically relevant changes in gene expression levels. In this study, we designed, constructed, and screened the synthetic regulatory parts by varying the promoter region and ribosome binding site element for V. natriegens with different transcriptional or translational strengths, respectively. The fluorescence intensities of the cells with different synthetic regulatory parts could distribute evenly over a wide range of 5 orders of magnitude. The selected synthetic regulatory parts had good stability in both nutrient-rich and minimal media. The precise combinatorial modulation of galP (GalP = galactose permease) and glk (Glk = glucokinase) from Escherichia coli by using three synthetic regulatory parts with different strengths was confirmed in a phosphoenolpyruvate:carbohydrate phosphotransferase system with inactive V. natriegens strain to alter the glucose transport. This work provides the simple, efficient, and standardized constitutive regulatory parts for V. natriegens synthetic biology. |
| WOS关键词 | EXPRESSION ; SEQUENCE ; PROTEIN ; TRANSLATION ; BACTERIUM ; PROMOTERS ; GLUCOSE |
| 资助项目 | National Natural Science Foundation of China[31700080] ; China Postdoctoral Science Foundation[2017M621082] ; Enterprise Postdoctoral Innovation Project of Tianjin[TJQYBSH2017006] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000574922400019 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; Enterprise Postdoctoral Innovation Project of Tianjin |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/42245]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Wang, Qinhong |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, CAS Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Fengli,Chen, Wujiu,Peng, Yanfeng,et al. Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology[J]. ACS SYNTHETIC BIOLOGY,2020,9(9):2399-2409. |
| APA | Wu, Fengli.,Chen, Wujiu.,Peng, Yanfeng.,Tu, Ran.,Lin, Yuping.,...&Wang, Qinhong.(2020).Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology.ACS SYNTHETIC BIOLOGY,9(9),2399-2409. |
| MLA | Wu, Fengli,et al."Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology".ACS SYNTHETIC BIOLOGY 9.9(2020):2399-2409. |
入库方式： OAI收割
来源：过程工程研究所
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