Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification
文献类型：期刊论文
| 作者 | Bao, Haiyan1; Li, Wei3; Yue, Xiqing1; Wu, Junrui1; Qiao, Yu4; Peng, Qing4; Shi, Bo4; Du, Yuguang2; Chen, Xu1; Wu, Rina1 |
| 刊名 | ANALYST
 |
| 出版日期 | 2020-10-21 |
| 卷号 | 145期号:20页码:6677-6682 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/d0an00965b |
| 英文摘要 | Bacillus cereus(B. cereus) is widespread in nature and considered an important foodborne pathogen, which can lead to emetic syndrome and diarrheal illness. Therefore, appropriate detection methods are needed to effectively monitor this pathogenic bacterium. Competitive annealing mediated isothermal amplification (CAMP) is a novel nucleic-acid-based detection technology that amplifies DNA with high sensitivity and specificity under isothermal conditions. The aim of this study was to develop a real-time CAMP assay for the rapid and simple detection ofB. cereusin milk. In this system, a pair of primers was designed to specifically target theentFMgene ofB. cereus. Compared with the conventional PCR method, the CAMP assay has higher sensitivity, the same specificity and shorter detection time. The detection limits of the CAMP assay for pure bacterial cultures and artificially contaminated milk samples were all 59 CFU mL(-1). And this detection method showed a wide linear range (from 5.9 x 10(5)to 59 CFU mL(-1)) and satisfactory recovery values (from 75.76% to 106.78%). These results indicate that the developed CAMP assay is a potentially useful method for the detection ofB. cereusin milk. |
| WOS关键词 | EMETIC BACILLUS-CEREUS ; ENTEROTOXIN GENES ; PROPIDIUM MONOAZIDE ; FOOD ; PCR ; STRAINS ; DIFFERENTIATION ; QUANTIFICATION ; ELISA ; ASSAY |
| 资助项目 | National Key R D Project[2018YFD0501403] ; Youth Project of the Education Department of Liaoning Province, China[LSNQN201911] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000576736000027 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | National Key R D Project ; Youth Project of the Education Department of Liaoning Province, China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/42330]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Chen, Xu; Wu, Rina |
| 作者单位 | 1.Shenyang Agr Univ, Food Sci Coll, Shenyang 110866, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 3.Shenyang Agr Univ, Dept Publicat, Shenyang 110866, Peoples R China 4.Chinese Acad Agr Sci, Feed Res Inst, Beijing 10008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, Haiyan,Li, Wei,Yue, Xiqing,et al. Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification[J]. ANALYST,2020,145(20):6677-6682. |
| APA | Bao, Haiyan.,Li, Wei.,Yue, Xiqing.,Wu, Junrui.,Qiao, Yu.,...&Wu, Rina.(2020).Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification.ANALYST,145(20),6677-6682. |
| MLA | Bao, Haiyan,et al."Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification".ANALYST 145.20(2020):6677-6682. |
入库方式： OAI收割
来源：过程工程研究所
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