Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus
文献类型：期刊论文
| 作者 | Sharshar, Moustafa Mohamed5,6; Samak, Nadia Abdrabou4,5,6; Ambreen, Sadaf3; Hao, Xuemi5,6; Mu, Tingzhen6; Maarouf, Mohamed1,2; Zheng, Chen5,6; Gao, Yibo5,6; Liu, Zhixia6; Jia, Yunpu5,6 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2020-12-01 |
| 卷号 | 317页码:11 |
| 关键词 | Nanometric sulfur bioproduction Desulfurization Cell death-induced CRISPR Sulfur pathways Thioalkalivibrio versutus |
| ISSN号 | 0960-8524 |
| DOI | 10.1016/j.biortech.2020.124018 |
| 英文摘要 | Complicated production procedures and superior characteristics of nano-sized sulfur elevate its price to 25-40 fold higher than micrograde kind. Also, natural gas hydrogen sulfide levels are restricted because of its toxic environmental consequences. Thioalkalivibrio versutus is a polyextremophilic industrial autotroph with high natural gas desulfurization capability. Here, nanometric ( > 50 nm) sulfur bioproduction using T. versutus while desulfurizing natural gas was validated. Also, this production was enhanced by 166.7% via lowering sulfate production by 55.1%. A specially-developed CRISPR system, with 42% editing efficiency, simplified the genome editing workflow scheme for this challenging bacterium. In parallel, sulfur metabolism was uncovered using proteins mining and transcriptome studies for defining sulfate-producing key genes (heterodisulfide reductase-like complex, sulfur dioxygenase, sulfite dehydrogenase and sulfite oxidase). This study provided cost-effective nanometric sulfur production and improved this production using a novel CRISPR strategy, which could be suitable for industrial polyextremophiles, after uncovering sulfur pathways in T. versutus. |
| WOS关键词 | FED-BATCH ; GENOME ; PERFORMANCE ; CRISPR ; OXIDATION ; SEQUENCE ; PLATFORM ; PCR |
| 资助项目 | National Science Foundation of China[21878307] ; National Science Foundation of China[31872633] ; National Science Foundation of China[31800030] ; National Key R&D Program of China[2020YFA0906800] ; CAS-TWAS (The World Academy of Sciences) |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| WOS记录号 | WOS:000577077400008 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | National Science Foundation of China ; National Key R&D Program of China ; CAS-TWAS (The World Academy of Sciences) |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/42409]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Xing, Jianmin |
| 作者单位 | 1.Suez Canal Univ, Fac Vet Med, Dept Virol, Ismailia 41522, Egypt 2.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China 4.Egyptian Petr Res Inst, Proc Design & Dev Dept, Cairo 11727, Egypt 5.Univ Chinese Acad Sci, Coll Chem Engn, 19 A Yuquan Rd, Beijing 100049, Peoples R China 6.Chinese Acad Sci, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Inst Proc Engn, Beijing 100190, Peoples R China 7.Chinese Acad Sci, Guangdong Prov Key Lab Synthet Genom, Shenzhen Key Lab Synthet Genom,Shenzhen Inst Adv, CAS Key Lab Quantitat Engn Biol,Shenzhen Inst Syn, Shenzhen 518055, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sharshar, Moustafa Mohamed,Samak, Nadia Abdrabou,Ambreen, Sadaf,et al. Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus[J]. BIORESOURCE TECHNOLOGY,2020,317:11. |
| APA | Sharshar, Moustafa Mohamed.,Samak, Nadia Abdrabou.,Ambreen, Sadaf.,Hao, Xuemi.,Mu, Tingzhen.,...&Xing, Jianmin.(2020).Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus.BIORESOURCE TECHNOLOGY,317,11. |
| MLA | Sharshar, Moustafa Mohamed,et al."Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus".BIORESOURCE TECHNOLOGY 317(2020):11. |
入库方式： OAI收割
来源：过程工程研究所
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