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Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System

文献类型：期刊论文

作者Gong, Mengge1,2; Li, Tiechuan5; Wu, Lina1; Zhang, Zhenxing4; Ren, Lishi1; Duan, Xuexin; Cao, Hongzhi3; Pei, Meishan2; Li, Jian-Jun1; Du, Yuguang1
刊名CATALYSTS
出版日期2020-11-01
卷号10期号:11页码:12
关键词biotinylated 6 '/3 '-sialyl-N-acetylglucosamine one-pot multienzyme solid-phase oligosaccharide synthesis influenza viruses ultrahigh-frequency-acoustofluidics
DOI10.3390/catal10111347
英文摘要6 '/3 '-Sialylated N-acetyllactosamine (6 '/3 '-SLN) is important for discrimination of the source (human or avian) of influenza virus strains. Biotinylated oligosaccharides have been widely used for analysis and quick detection. The development of efficient strategies to synthesize biotin-tagged 6 '/3 '-SLN have become necessary. Effective mixing is essential for enzymatic solid-phase oligosaccharide synthesis (SPOS). In the current study, newly developed technology ultrahigh-frequency-acoustofluidics (UHFA), which can provide a powerful source for efficient microfluidic mixing, solid-phase oligosaccharide synthesis and one-pot multienzyme (OPME) system, were used to develop a new strategy for oligosaccharide synthesis. Firstly, biotinylated N-acetylglucosamine was designed and chemically synthesized through traditional approaches. Secondly, biotinylated 6 '- and 3 '-sialyl-N-acetylglucosamines were prepared in solution through two sequential OPME modules in with a yield of similar to 95%. Thirdly, 6 '-SLN was also prepared through UHFA-based enzymatic solid-phase synthesis on magnetic beads with a yield of 64.4%. The current strategy would be potentially used for synthesis of functional oligosaccharides.
WOS关键词EFFICIENT CHEMOENZYMATIC SYNTHESIS ; ACID ; OLIGOSACCHARIDE ; TETRASACCHARIDE ; STREPTAVIDIN ; AFFINITY ; GLYCANS ; CANCER ; ROLES ; CELLS
资助项目National Natural Science Foundation of China[21877114]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000593655800001
出版者MDPI
资助机构National Natural Science Foundation of China
源URL[http://ir.ipe.ac.cn/handle/122111/42729]  
专题中国科学院过程工程研究所
通讯作者Pei, Meishan; Li, Jian-Jun
作者单位1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Natl Engn Res Ctr Biotechnol Beijing, PLA,Inst Proc Engn,Key Lab Biopharmaceut Prod & F, Beijing 100190, Peoples R China
2.Univ Jinan, Sch Chem & Chem Engn, Jinan 250022, Peoples R China
3.Shandong Univ, State Key Lab Microbial Technol, Natl Glycoengn Res Ctr, Qingdao 266237, Peoples R China
4.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China
5.Tianjin Univ, State Key Lab Precis Measuring Technol & Instrume, Tianjin 300072, Peoples R China
推荐引用方式
GB/T 7714		 Gong, Mengge,Li, Tiechuan,Wu, Lina,et al. Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System[J]. CATALYSTS,2020,10(11):12.
APA Gong, Mengge.,Li, Tiechuan.,Wu, Lina.,Zhang, Zhenxing.,Ren, Lishi.,...&Du, Yuguang.(2020).Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System.CATALYSTS,10(11),12.
MLA Gong, Mengge,et al."Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System".CATALYSTS 10.11(2020):12.

入库方式： OAI收割

来源：过程工程研究所

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