Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix
文献类型：期刊论文
| 作者 | Li, Ke2,3; Cao, Y-x2; Jiao, S-m3; Du, G-h1; Du, Y-g3; Qin, X-m2 |
| 刊名 | FRONTIERS IN PHARMACOLOGY
 |
| 出版日期 | 2020-11-24 |
| 卷号 | 11页码:18 |
| 关键词 | Astragalus polysaccharide different molecular weight immunomodulatory activity nonspecific and specific structure characterization |
| ISSN号 | 1663-9812 |
| DOI | 10.3389/fphar.2020.582091 |
| 英文摘要 | Saccharides are the most abundant substance with the strongest immunological activity in Astragali Radix (AR). However, systematic structure study and immunoactivity screening of polysaccharides with different molecular weights (Mw) in AR have yet to be conducted. In this study, Astragalus polysaccharides (APSs) were divided into three fragments of different Mw values, >2,000 kDa (APS-?), about 10 kDa (APS-II), and about 300 Da (APS-I), by using ultrafiltration for the first time. The structural differences of the three products were determined on the basis of monosaccharide composition, FT-IR spectrum, linkage analysis, and nuclear magnetic resonance analysis. Cellular immune activity experiments in vitro and cyclophosphamide immunosuppression animal model experiments in vivo for nonspecific and specific immunoactivity screening were applied to identify the most immunogenic fragment in APSs. Linkage analysis results showed that APS-?, APS-II, and APS-III have different attachment sites of monosaccharide residues. Immune screening experiments indicated that the Mw of the APSs influenced their activity, and APS-II had the strongest immunoenhancing activity among the products. This research may serve as a reference for further study on APSs with different structures and immune activities, and as a guidance for the quality control of APSs and the development of new APS products. |
| WOS关键词 | ANTIOXIDANT ACTIVITY |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000596267100001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/42803]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Du, Y-g; Qin, X-m |
| 作者单位 | 1.Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, Beijing, Peoples R China 2.Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ke,Cao, Y-x,Jiao, S-m,et al. Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix[J]. FRONTIERS IN PHARMACOLOGY,2020,11:18. |
| APA | Li, Ke,Cao, Y-x,Jiao, S-m,Du, G-h,Du, Y-g,&Qin, X-m.(2020).Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix.FRONTIERS IN PHARMACOLOGY,11,18. |
| MLA | Li, Ke,et al."Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix".FRONTIERS IN PHARMACOLOGY 11(2020):18. |
入库方式： OAI收割
来源：过程工程研究所
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