pH-sensitive zwitterionic polycarboxybetaine as a potential non-viral vector for small interfering RNA delivery
文献类型：期刊论文
| 作者 | Peng, Huan1,2; Ji, Weihong1,2; Zhao, Ruichen1,2; Lu, Zhiguo1,2; Yang, Jun1; Li, Yan1 ; Zhang, Xin1
|
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2020-12-20 |
| 卷号 | 10期号:73页码:45059-45066 |
| DOI | 10.1039/d0ra09359a |
| 英文摘要 | Small interfering RNA (siRNA) has great potential for the treatment of various diseases. However, its intrinsic deficiencies seriously limit its application. Herein, pH-sensitive zwitterionic polymer polycarboxybetaine (PCB) was developed as a non-viral vector for siRNA. The PCB could be protonated in an acidic environment and become positively charged from a cancer site. After protonation, PCB could complex siRNA via electrostatic interaction, and its loading ability was enhanced with a decrease of pH value. Compared with the PEI 10k, PCB50 with a similar molecular weight had comparable siRNA loading ability and lower cytotoxicity. Besides, siRNA loaded by PCB50 could escape from endosomes and reduce the loss of drugs, and based on the excellent uptake and obvious apoptotic effect on HeLa cells, the pH-sensitive PCB with low cytotoxicity could be used as a non-viral vector for safe siRNA delivery for cancer treatment. |
| WOS关键词 | IN-VIVO ; SIRNA ; GENE ; POLYETHYLENIMINE ; THERAPEUTICS ; POLYMER ; LIPOSOMES ; SERUM |
| 资助项目 | Beijing Natural Science Foundation[L172046] ; Beijing Natural Science Foundation[2192057] ; Beijing Nova Program[Z201100006820140] ; National High Technology Research and Development Program[2016YFA0200303] ; National Natural Science Foundation of China[31771095] ; National Natural Science Foundation of China[21875254] ; National Natural Science Foundation of China[21905283] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000601124900049 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | Beijing Natural Science Foundation ; Beijing Nova Program ; National High Technology Research and Development Program ; National Natural Science Foundation of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/42907]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Li, Yan; Zhang, Xin |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Peng, Huan,Ji, Weihong,Zhao, Ruichen,et al. pH-sensitive zwitterionic polycarboxybetaine as a potential non-viral vector for small interfering RNA delivery[J]. RSC ADVANCES,2020,10(73):45059-45066. |
| APA | Peng, Huan.,Ji, Weihong.,Zhao, Ruichen.,Lu, Zhiguo.,Yang, Jun.,...&Zhang, Xin.(2020).pH-sensitive zwitterionic polycarboxybetaine as a potential non-viral vector for small interfering RNA delivery.RSC ADVANCES,10(73),45059-45066. |
| MLA | Peng, Huan,et al."pH-sensitive zwitterionic polycarboxybetaine as a potential non-viral vector for small interfering RNA delivery".RSC ADVANCES 10.73(2020):45059-45066. |
入库方式： OAI收割
来源：过程工程研究所
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