Intracellular Paclitaxel Delivery Facilitated by a Dual-Functional CPP with a Hydrophobic Hairpin Tail
文献类型：期刊论文
| 作者 | Wei, Yuping1,2; Zhang, Man3; Jiao, Pengfei2; Zhang, Xin2; Yang, Ganggang4,5; Xu, Xia1,4,5 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2021-02-03 |
| 卷号 | 13期号:4页码:4853-4860 |
| 关键词 | dual-functional peptide cell penetrating peptide paclitaxel intracellular drug delivery double-proline hairpin tail |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.0c20180 |
| 英文摘要 | In our pervious study, a dual-functional peptide R7 was developed to form a complex with paclitaxel (PTX) for enhancement of PTX translocation. However, because of the unstable noncovalent bond between R7 and PTX, PTX redistributed after the introduction of heparin, leading to R7-PTX complex dissociation, further causing less PTX penetration than expected. Thus, a novel positive CPP carrier of P9 was developed to improve CPP-PTX affinity via a double-proline (Pro, P) hairpin tail and enhance PTX translocation through the reduction of translocation energy barrier, confirmed by the MM-PBSA analysis and umbrella sampling simulation. Cellular uptake study reveals that P9 can quickly translocate into the HeLa cells within 1 min and exhibits no noticeable cytotoxicity. Compared to R7, P9 is able to help PTX translocation, leading to a remarkable increase in the intracellular concentration of PTX, eventually resulting in a significant loss in tumor cell viability. In vivo experiments demonstrate that a vein injection of P9-PTX complex dramatically inhibits tumor growth. Our study provides a novel perspective for designing CPP-facilitated drug carrier to enhance antitumor efficiency. |
| WOS关键词 | PEPTIDES |
| 资助项目 | National Natural Science Foundation of China[21506230] ; National Natural Science Foundation of China[21978001] ; National Natural Science Foundation of China[21606239] ; National Natural Science Foundation of China[31770138] ; National Natural Science Foundation of China[31570120] ; Wanjiang Scholar Program and Start Fund from Anhui University of Technology ; Scientific and Technological Project of Henan Province[202102310537] ; School-level Research Project of Nanyang Normal University[2019ZX012] |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000618153100004 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Science Foundation of China ; Wanjiang Scholar Program and Start Fund from Anhui University of Technology ; Scientific and Technological Project of Henan Province ; School-level Research Project of Nanyang Normal University |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/43443]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Xu, Xia |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Nanyang Normal Univ, Sch Life Sci & Technol, Nanyang 473061, Henan, Peoples R China 3.Nanyang First Peoples Hosp, Dept Oncol, Nanyang 473002, Henan, Peoples R China 4.Anhui Univ Technol, Biochem Engn Res Ctr, Maanshan 243032, Henan, Peoples R China 5.Anhui Univ Technol, Sch Chem & Chem Engn, Maanshan 243032, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Yuping,Zhang, Man,Jiao, Pengfei,et al. Intracellular Paclitaxel Delivery Facilitated by a Dual-Functional CPP with a Hydrophobic Hairpin Tail[J]. ACS APPLIED MATERIALS & INTERFACES,2021,13(4):4853-4860. |
| APA | Wei, Yuping,Zhang, Man,Jiao, Pengfei,Zhang, Xin,Yang, Ganggang,&Xu, Xia.(2021).Intracellular Paclitaxel Delivery Facilitated by a Dual-Functional CPP with a Hydrophobic Hairpin Tail.ACS APPLIED MATERIALS & INTERFACES,13(4),4853-4860. |
| MLA | Wei, Yuping,et al."Intracellular Paclitaxel Delivery Facilitated by a Dual-Functional CPP with a Hydrophobic Hairpin Tail".ACS APPLIED MATERIALS & INTERFACES 13.4(2021):4853-4860. |
入库方式： OAI收割
来源：过程工程研究所
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