中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 过程工程研究所
  3. 中国科学院过程工程研究所
On-line separation and quantification of virus antigens of different serotypes in multivalent vaccines by capillary zone electrophoresis: A case study for quality control of foot-and-mouth disease virus vaccines

文献类型：期刊论文

作者Song, Yanmin1,2; Yang, Yanli1; Lin, Xuan1; Zhao, Qizu3; Li, Zhengjun1; Ma, Guanghui1; Su, Zhiguo1; Zhang, Songping1
刊名JOURNAL OF CHROMATOGRAPHY A
出版日期2021-01-25
卷号1637页码:8
关键词Capillary zone electrophoresis FMDV Quantitation Serotyping Quality control
ISSN号0021-9673
DOI10.1016/j.chroma.2020.461834
英文摘要Accurate quantification of effective antigens of different serotypes is crucial for quality control of multivalent vaccines but challenging. A simple and rapid capillary zone electrophoresis (CZE) method was developed for on-line separation and quantification of foot-and-mouth disease virus (FMDV) antigens in monovalent and bivalent FMDV vaccines. The FMDV peak identity in CZE was demonstrated by the study of FMDV dissociation combined with high performance size exclusion chromatography (HPSEC) analysis. After optimizing CZE conditions including UV detecting wavelength, injection volume, and separation voltage, both serotype A and O FMDV showed good reproducibility (RSD <5%) and linear responses (R-2 =0.999) between the peak area and FMDV content in the concentration range of 15-400 mu g/mL. The two serotypes of FMDV with similar size had different migration time in CZE according to their different zeta potential, which allows them to be separated and quantified, with accuracy of <10% relative error. CZE was then successfully applied for antigen quantification of commercial O monovalent and A/O bivalent FMDV vaccines. Compared with HPSEC, CZE was not only able to quantify each serotype of FMDV, but also free from interference of nucleic acids impurities. In summary, the CZE can be a simple, rapid, and reliable tool for quality control of monovalent and bivalent FMDV vaccines. The CZE method can also be further extended to the quality control of other multivalent virus and virus like particle vaccines. (C) 2020 Elsevier B.V. All rights reserved.
资助项目National Natural Science Foundation of China[21808226] ; National Natural Science Foundation of China[31970872] ; National Natural Science Foundation of China[21821005]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000616104700001
出版者ELSEVIER
资助机构National Natural Science Foundation of China
源URL[http://ir.ipe.ac.cn/handle/122111/43465]  
专题中国科学院过程工程研究所
通讯作者Yang, Yanli; Zhang, Songping
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China
3.China Inst Vet Drug Control, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714		 Song, Yanmin,Yang, Yanli,Lin, Xuan,et al. On-line separation and quantification of virus antigens of different serotypes in multivalent vaccines by capillary zone electrophoresis: A case study for quality control of foot-and-mouth disease virus vaccines[J]. JOURNAL OF CHROMATOGRAPHY A,2021,1637:8.
APA Song, Yanmin.,Yang, Yanli.,Lin, Xuan.,Zhao, Qizu.,Li, Zhengjun.,...&Zhang, Songping.(2021).On-line separation and quantification of virus antigens of different serotypes in multivalent vaccines by capillary zone electrophoresis: A case study for quality control of foot-and-mouth disease virus vaccines.JOURNAL OF CHROMATOGRAPHY A,1637,8.
MLA Song, Yanmin,et al."On-line separation and quantification of virus antigens of different serotypes in multivalent vaccines by capillary zone electrophoresis: A case study for quality control of foot-and-mouth disease virus vaccines".JOURNAL OF CHROMATOGRAPHY A 1637(2021):8.

入库方式： OAI收割

来源：过程工程研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。