胸腺素α1的DIC固相化学合成与鉴定
文献类型：期刊论文
| 作者 | 甘一如1; 黄永东1; 刘晋生1; 张雪竹1; 张贵峰2 |
| 刊名 | 化工学报
 |
| 出版日期 | 2004 |
| 卷号 | 55.0期号:002页码:305 |
| 关键词 | 胸腺素α1 固相多肽合成 二异丙基碳二亚胺（DIC） 反相高效液相色谱法 聚丙烯酰胺凝胶电泳 （SDS-PAGE）法 液相色谱／质谱（LC／MS） |
| ISSN号 | 0438-1157 |
| 英文摘要 | Thymosin α1 was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α1 was synthesized with the solid-phase method, by using the base-liable Fmoc group to protect the a-amino acid and N,N'-diisopropylcarbodiimide (DIC) as the coupling regent. The crude product was analyzed by reversed-phase high performance liquid chromatography, SDS-PAGE and liquid chromatography/mass spectroscopy. The synthesis yield was 84.89%. The analytical results indicated that the crude product contained much thymosin α1, and the purity was 50.3%. DIC solid-phase chemical synthesis of thymosin α1 was applicable, and the yield was improved greatly compared to other known methods. |
| 语种 | 英语 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/45042]  |
| 专题 | 中国科学院过程工程研究所 |
| 作者单位 | 1.天津大学 2.中国科学院过程工程研究所 |
| 推荐引用方式 GB/T 7714 | 甘一如,黄永东,刘晋生,等. 胸腺素α1的DIC固相化学合成与鉴定[J]. 化工学报,2004,55.0(002):305. |
| APA | 甘一如,黄永东,刘晋生,张雪竹,&张贵峰.(2004).胸腺素α1的DIC固相化学合成与鉴定.化工学报,55.0(002),305. |
| MLA | 甘一如,et al."胸腺素α1的DIC固相化学合成与鉴定".化工学报 55.0.002(2004):305. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。