E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity
文献类型：期刊论文
| 作者 | Huang DD(黄丹丹)1,2,3; Ding QH(丁奇寒)1,2,3; Chen SB(陈深宝)1,2,3 ; Lv SQ(吕守芹)1,2,3; Zhang Y(章燕)1,2,3; Long M(龙勉)1,2,3
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| 刊名 | FASEB JOURNAL
 |
| 出版日期 | 2021-05-01 |
| 卷号 | 35期号:5页码:15 |
| 关键词 | cytoskeletal remodeling endothelial integrity E-selectin neutrophil transmigration |
| ISSN号 | 0892-6638 |
| DOI | 10.1096/fj.202000662RR |
| 通讯作者 | Zhang, Yan(zhangyan@imech.ac.cn) ; Long, Mian(mlong@imech.ac.cn) |
| 英文摘要 | Transendothelial migration (TEM) of neutrophils under blood flow is critical in the inflammatory cascade. However, the role of endothelial plasticity in this process is not fully understood. Therefore, we used an in vitro model to test the dynamics of human polymorphonuclear neutrophil (PMN) TEM across lipopolysaccharide-treated human umbilical vein endothelial cell (HUVEC) monolayers. Interestingly, shRNA-E-selectin knockdown in HUVECs destabilized endothelial junctional integrity by reducing actin branching and increasing stress fiber at cell-cell junctions. This process is accomplished by downregulating the activation of cortactin and Arp2/3, which in turn alters the adhesive function of VE-cadherin, enhancing PMN transmigration. Meanwhile, redundant P-selectins possess overlapping functions in E-selectin-mediated neutrophil adhesion, and transmigration. These results demonstrate, to our knowledge, for the first time, that E-selectins negatively regulate neutrophil transmigration through alterations in endothelial plasticity. Furthermore, it improves our understanding of the mechanisms underlying actin remodeling, and junctional integrity, in endothelial cells mediating leukocyte TEM. |
| 分类号 | 二类/Q1 |
| WOS关键词 | ARP2/3 COMPLEX ; CELL-ADHESION ; LEUKOCYTE ADHESION ; ACTIN ; DYNAMICS ; LAMELLIPODIA ; CORTACTIN ; MOLECULES ; MIGRATION ; PROTEINS |
| 资助项目 | National Natural Science Foundation of China (NSFC)[31627804] ; National Natural Science Foundation of China (NSFC)[91642203] ; National Natural Science Foundation of China (NSFC)[11772345] ; National Natural Science Foundation of China (NSFC)[91539119] ; National Key Rsearch and Development Program of China[2016YFA0501601] ; Chinese Academy of Sciences Strategic Priority Research Program[XDB22040101] ; Frontier Science Key Project[QYZDJ--SSW--JSC018] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000645260100005 |
| 资助机构 | National Natural Science Foundation of China (NSFC) ; National Key Rsearch and Development Program of China ; Chinese Academy of Sciences Strategic Priority Research Program ; Frontier Science Key Project |
| 其他责任者 | Zhang, Yan ; Long, Mian |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/86895]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China; 3.Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Ctr Biomech & Bioengn,Key Lab Micrograv, Beijing 100190, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Huang DD,Ding QH,Chen SB,et al. E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity[J]. FASEB JOURNAL,2021,35(5):15. |
| APA | 黄丹丹,丁奇寒,陈深宝,吕守芹,章燕,&龙勉.(2021).E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity.FASEB JOURNAL,35(5),15. |
| MLA | 黄丹丹,et al."E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity".FASEB JOURNAL 35.5(2021):15. |
入库方式： OAI收割
来源：力学研究所
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