Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells
文献类型：期刊论文
| 作者 | Qin, Feng4,5,6,7; Fan, Qiang4,5; Yu, Peter K. N.8,9; Almahi, Waleed Abdelbagi4,5; Kong, Peizhong4,5; Yang, Miaomiao2,4,5; Cao, Wei4,5; Nie, Lili4,6 ; Chen, Guodong4,6; Han, Wei1,3,4,6
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| 刊名 | ANNALS OF TRANSLATIONAL MEDICINE
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| 出版日期 | 2021-04-01 |
| 卷号 | 9 |
| 关键词 | Breast cancer acquired radioresistance gene expression profiling energy metabolism |
| ISSN号 | 2305-5839 |
| DOI | 10.21037/atm-20-4667 |
| 通讯作者 | Chen, Guodong(c3024034@163.com) ; Han, Wei(hanw@hfcas.ac.cn) |
| 英文摘要 | Background: Acquired radioresistant cells exhibit many characteristic changes which may influence cancer progression and further treatment options. The purpose of this study is to investigate the changes of radioresistant human epidermal growth factor receptor 2 (HER2)-positive breast cancer cells on both phenotypic and molecular levels. Methods: We established an acquired radioresistant cell line from its parental NF639 cell line (HER2-positive) by fractionated radiation and assessed changes in cellular morphology, proliferation, migration, anti-apoptosis activity, basal reactive oxygen species (ROS) level and energy metabolism. RNA-sequencing (RNA-seq) was also used to reveal the potential regulating genes and molecular mechanisms associated with the acquired changed phenotypes. Real-time PCR was used to validate the results of RNA-seq. Results: The NF639R cells exhibited increased radioresistance and enhanced activity of proliferation, migration and anti-apoptosis, but decreased basal ROS. Two main energy metabolism pathways, mitochondrial respiration and glycolytic, were also upregulated. Furthermore, 490 differentially expressed genes were identified by RNA-seq. Enrichment analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes showed many differently expressed genes were significantly enriched in cell morphology, proliferation, migration, anti-apoptosis, antioxidation, tumor stem cells and energy metabolism and the signaling cascades such as the transforming growth factor-beta, Wnt, Hedgehog, vascular endothelial growth factor, retinoic acid-inducible gene I (RIG-I)-like receptor, Toll-like receptor and nucleotide oligomerization domain (NOD)-like receptor were significantly altered in NF639R cells. Conclusions: In clinical radiotherapy, repeat radiotherapy for short-term recurrence of breast cancer may result in enhanced radioresistance and promote malignant progression. Our research provided hints to understand the tumor resistance to radiotherapy de novo and recurrence with a worse prognosis following radiotherapy. Keywords: |
| WOS关键词 | EPITHELIAL-MESENCHYMAL TRANSITION ; PATTERN-RECOGNITION RECEPTORS ; TUMOR-CELLS ; CELLULAR-RESPONSES ; ENERGY-METABOLISM ; DOSE RADIATION ; GROWTH ; CARCINOMA ; EMT ; PROLIFERATION |
| 资助项目 | Chinese National Natural Science Foundation[81703168] ; Chinese National Natural Science Foundation[U1632145] ; Chinese National Natural Science Foundation[81227902] ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2019HSCCIP010] ; Natural Science Fund of Anhui Province[1608085QH181] ; PriorityAcademic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Laboratory in Marine Pollution, City University of Hong Kong[IRF/0024] |
| WOS研究方向 | Oncology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000647719000025 |
| 出版者 | AME PUBL CO |
| 资助机构 | Chinese National Natural Science Foundation ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Natural Science Fund of Anhui Province ; PriorityAcademic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Laboratory in Marine Pollution, City University of Hong Kong |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/122152]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Chen, Guodong; Han, Wei |
| 作者单位 | 1.Soochow Univ, Collaborat Innovat Ctr Radiat Med, Jiangsu Higher Educ Inst, Suzhou, Peoples R China 2.Anhui Med Univ, Affiliated Hosp 4, Clin Pathol Ctr, Hefei, Peoples R China 3.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou, Peoples R China 4.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei, Peoples R China 5.Grad Sch USTC, Sci Isl Branch, Hefei, Peoples R China 6.Chinese Acad Sci, Hefei Canc Hosp, Hefei, Peoples R China 7.Anhui Acad Agr Sci, Inst Sericultural, Hefei, Peoples R China 8.City Univ Hong Kong, Dept Phys, Kowloon Tong, Tat Chee Ave, Hong Kong, Peoples R China 9.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon Tong, Tat Chee Ave, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Feng,Fan, Qiang,Yu, Peter K. N.,et al. Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells[J]. ANNALS OF TRANSLATIONAL MEDICINE,2021,9. |
| APA | Qin, Feng.,Fan, Qiang.,Yu, Peter K. N..,Almahi, Waleed Abdelbagi.,Kong, Peizhong.,...&Han, Wei.(2021).Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells.ANNALS OF TRANSLATIONAL MEDICINE,9. |
| MLA | Qin, Feng,et al."Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells".ANNALS OF TRANSLATIONAL MEDICINE 9(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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