Development of a Vaccine against SARS-CoV-2 Based on the Receptor-Binding Domain Displayed on Virus-Like Particles
文献类型：期刊论文
| 作者 | Zha, Lisha4; Chang, Xinyue5,6; Zhao, Hongxin7 ; Mohsen, Mona O.5,6,8; Hong, Liang4; Zhou, Yuhang9; Chen, Hongquan4; Liu, Xuelan4,5; Zhang, Jie9; Li, Dong10
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| 刊名 | VACCINES
 |
| 出版日期 | 2021-04-01 |
| 卷号 | 9 |
| 关键词 | COVID-19 vaccine virus-like particle CuMVTT-RBD BLI |
| DOI | 10.3390/vaccines9040395 |
| 通讯作者 | Wang, Junfeng(junfeng@hmfl.ac.cn) ; Bachmann, Martin F.(martin.bachmann@dbmr.unibe.ch) |
| 英文摘要 | The ongoing coronavirus disease (COVID-19) pandemic is caused by a new coronavirus (severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2)) first reported in Wuhan City, China. From there, it has been rapidly spreading to many cities inside and outside China. Nowadays, more than 110 million cases with deaths surpassing 2 million have been recorded worldwide, thus representing a major health and economic issues. Rapid development of a protective vaccine against COVID-19 is therefore of paramount importance. Here, we demonstrated that the recombinantly expressed receptor-binding domain (RBD) of the spike protein can be coupled to immunologically optimized virus-like particles derived from cucumber mosaic virus (CuMVTT). The RBD displayed CuMVTT bound to ACE2, the viral receptor, demonstrating proper folding of RBD. Furthermore, a highly repetitive display of the RBD on CuMVTT resulted in a vaccine candidate that induced high levels of specific antibodies in mice, which were able to block binding of the spike protein to ACE2 and potently neutralize SARS-CoV-2 virus in vitro. |
| 资助项目 | Saiba AG ; Swiss National Science Foundation (SNF)[31003A_185114] ; International Immunology Centre, Anhui Agricultural University, Hefei, China |
| WOS研究方向 | Immunology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000643781900001 |
| 出版者 | MDPI |
| 资助机构 | Saiba AG ; Swiss National Science Foundation (SNF) ; International Immunology Centre, Anhui Agricultural University, Hefei, China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/122161]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Wang, Junfeng; Bachmann, Martin F. |
| 作者单位 | 1.Artemis Reserch One Heath Fdn, NL-7100 Delft, Netherlands 2.Southern Univ Sci & Technol, Acad Interdisciplinary & Adv Studies, Inst Risk Anal Predict & Management, Shenzhen 518055, Peoples R China 3.Univ Oxford, Jenner Inst, Old Rd Campus,Roosevelt Dr, Oxford OX3 7BN, England 4.Anhui Agr Univ, Int Immunol Ctr, Hefei 230036, Peoples R China 5.Univ Hosp Bern, Dept Rheumatol & Immunol, CH-3010 Bern, Switzerland 6.Univ Bern, Dept BioMed Res, CH-3012 Bern, Switzerland 7.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Peoples R China 8.Saiba AG, CH-8808 Pfaffikon, Switzerland 9.Shandong H&Z Lifesci Gmbh, Yantai 264000, Peoples R China 10.Beijing Key Lab Monoclonal Antibody Res & Dev, Beijing 100176, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zha, Lisha,Chang, Xinyue,Zhao, Hongxin,et al. Development of a Vaccine against SARS-CoV-2 Based on the Receptor-Binding Domain Displayed on Virus-Like Particles[J]. VACCINES,2021,9. |
| APA | Zha, Lisha.,Chang, Xinyue.,Zhao, Hongxin.,Mohsen, Mona O..,Hong, Liang.,...&Bachmann, Martin F..(2021).Development of a Vaccine against SARS-CoV-2 Based on the Receptor-Binding Domain Displayed on Virus-Like Particles.VACCINES,9. |
| MLA | Zha, Lisha,et al."Development of a Vaccine against SARS-CoV-2 Based on the Receptor-Binding Domain Displayed on Virus-Like Particles".VACCINES 9(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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