中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 合肥物质科学研究院
  3. 中国科学院合肥物质科学研究院
Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus

文献类型：期刊论文

作者Zhong, Zhuoheng2,6; Liu, Shengzhi2; Han, Songling2; Li, Yaohan2; Tao, Minglei2; Liu, Amin2; He, Qiang1; Chen, Sixue3; Dufresne, Criag5; Zhu, Wei4,6
刊名INDUSTRIAL CROPS AND PRODUCTS
出版日期2021-08-01
卷号166
ISSN号0926-6690
关键词Mitochondrial respiratory complex Catharanthus roseus Ultraviolet B Alkaloids
DOI10.1016/j.indcrop.2021.113448
通讯作者Zhu, Wei(zhuwei2007100@163.com) ; Tian, Jingkui(tjk@zju.edu.cn)
英文摘要Indole alkaloids from Catharanthus roseus (L.) G. Don were valued for their wide spectrum of pharmaceutical effects. Their biosynthesis is modulated by various abiotic factors including ultraviolet B radiation which induced ATP production and led to accumulation of indole alkaloids. To investigate the regulatory mechanism of secondary metabolism in the leaf mitochondria of C. roseus under ultraviolet B radiation, combinatory study of proteomic and metabolomic analyses of C. roseus was performed. ATP export in the leaves of C. roseus increased under UVB radiation, which required mitochondrial ATP synthase. Proteins related to mitochondrial complexes II/IV and their gene expression levels increased, and those related to mitochondrial complex I decreased. Proteins related to the methylerythritol phosphate pathway, especially geranylgeranyl pyrophosphate synthase, increased under ultraviolet B radiation. Metabolites whose levels changed were mainly alkaloids, organic acids, carbohydrates, phenylpropanoids, and fatty acids, while eight indole alkaloids increased. Integrative analysis of omics data indicated that the metabolism of glutamate and tyrosine was downregulated. These results suggest that ultraviolet B radiation induces dynamic changes in mitochondria in C. roseus, which guarantees ATP production, regulates the flux of the methylerythritol phosphate pathway to the biosynthesis of monoterpene moieties, and leads to accumulation of various indole alkaloids.
WOS关键词MITOCHONDRIAL ; BIOSYNTHESIS ; METABOLISM ; RESPIRATION ; PROTEOMICS ; STRESS ; ATP ; ORGANIZATION ; PROTEINS ; SYNTHASE
资助项目National Natural Science Foundation of China[81872973] ; National Science and Technology Major project of China[2019ZX09301108] ; Jiangsu Provincial Natural Science Foundation of China[BK20190223]
WOS研究方向Agriculture
语种英语
出版者ELSEVIER
WOS记录号WOS:000649652800003
资助机构National Natural Science Foundation of China ; National Science and Technology Major project of China ; Jiangsu Provincial Natural Science Foundation of China
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/122263]  
专题中国科学院合肥物质科学研究院
通讯作者Zhu, Wei; Tian, Jingkui
作者单位1.Zhejiang Prov Peoples Hosp, Dept Nephrol, Hangzhou 310014, Peoples R China
2.Zhejiang Univ, Coll Biomed Engn & Instrument Sci, Hangzhou 310027, Peoples R China
3.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Dept Biol Prote & Mass Spectrometry, Gainesville, FL 32610 USA
4.Changshu Qiushi Technol Co Ltd, Suzhou 215500, Peoples R China
5.Thermo Fisher Sci, W Palm Beach, FL 33407 USA
6.Univ Chinese Acad Sci, Canc Hosp, Zhejiang Canc Hosp, Inst Canc & Basic Med,Chinese Acad Sci, Hangzhou 310022, Peoples R China
推荐引用方式
GB/T 7714		 Zhong, Zhuoheng,Liu, Shengzhi,Han, Songling,et al. Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus[J]. INDUSTRIAL CROPS AND PRODUCTS,2021,166.
APA Zhong, Zhuoheng.,Liu, Shengzhi.,Han, Songling.,Li, Yaohan.,Tao, Minglei.,...&Tian, Jingkui.(2021).Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus.INDUSTRIAL CROPS AND PRODUCTS,166.
MLA Zhong, Zhuoheng,et al."Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus".INDUSTRIAL CROPS AND PRODUCTS 166(2021).

入库方式： OAI收割

来源：合肥物质科学研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。