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Architecture of the mycobacterial succinate dehydrogenase with a membrane-embedded Rieske FeS cluster

文献类型：期刊论文

作者Zhou, Xiaoting6,7,8,9,10; Gao, Yan6,7; Wang, Weiwei6,7,9,10; Yang, Xiaolin6,7; Yang, Xiuna6,7; Liu, Fengjiang6,7,9,10; Tang, Yanting8; Lam, Sin Man1; Shui, Guanghou1; Yu, Lu5
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2021-04-13
卷号118
ISSN号0027-8424
关键词succinate dehydrogenase Mycobacterium smegmatis electron transport chain cryoelectron microscopy
DOI10.1073/pnas.2022308118
通讯作者Wang, Quan(wangq@shanghaitech.edu.cn) ; Rao, Zihe(raozh@mail.tsinghua.edu.cn) ; Gong, Hongri(gonghr@nankai.edu.cn)
英文摘要Complex II, also known as succinate dehydrogenase (SQR) or fumarate reductase (QFR), is an enzyme involved in both the Krebs cycle and oxidative phosphorylation. Mycobacterial Sdh1 has recently been identified as a new class of respiratory complex II (type F) but with an unknown electron transfer mechanism. Here, using cryoelectron microscopy, we have determined the structure of Mycobacterium smegmatis Sdh1 in the presence and absence of the substrate, ubiquinone-1, at 2.53-angstrom and 2.88-angstrom resolution, respectively. Sdh1 comprises three subunits, two that arewater soluble, SdhA and SdhB, and one that is membrane spanning, SdhC. Within these subunits we identified a quinone-binding site and a rarely observed Rieske-type [2Fe-2S] cluster, the latter being embedded in the transmembrane region. A mutant, where two His ligands of the Rieske-type [2Fe-2S] were changed to alanine, abolished the quinone reduction activity of the Sdh1. Our structures allow the proposal of an electron transfer pathway that connects the substrate-binding and quinone-binding sites. Given the unique features of Sdh1 and its essential role in Mycobacteria, these structures will facilitate antituberculosis drug discovery efforts that specifically target this complex.
WOS关键词COMPLEX-II ; UBIQUINONE OXIDOREDUCTASE ; QUINOLFUMARATE REDUCTASE ; FUMARATE REDUCTASE ; ELECTRON-TRANSFER ; CRYSTAL-STRUCTURE ; HEME ; INTERMEDIATE ; BIOLOGY ; SITE
资助项目National Key Research and Development Program of China[2017YFC0840300] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB37030201] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB37040000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB37020203] ; National Natural Science Foundation of China[81520108019] ; National Natural Science Foundation of China[813300237] ; Tianjin Natural Science Foundation[20JCQNJC01430]
WOS研究方向Science & Technology - Other Topics
语种英语
出版者NATL ACAD SCIENCES
WOS记录号WOS:000641174100007
资助机构National Key Research and Development Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Tianjin Natural Science Foundation
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/122282]  
专题中国科学院合肥物质科学研究院
通讯作者Wang, Quan; Rao, Zihe; Gong, Hongri
作者单位1.Chinese Acad Sci, State Key Lab Mol Dev Biol, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
2.Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
4.Chinese Acad Sci, Natl Lab Biomacromol, CAS Ctr Excellence Biomacromol, Inst Biophys, Beijing 100101, Peoples R China
5.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Peoples R China
6.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China
7.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
8.Nankai Univ, State Key Lab Med Chem Biol, Frontiers Sci Ctr Cell Responses, Coll Life Sci, Tianjin 300353, Peoples R China
9.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
10.Univ CAS, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Xiaoting,Gao, Yan,Wang, Weiwei,et al. Architecture of the mycobacterial succinate dehydrogenase with a membrane-embedded Rieske FeS cluster[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2021,118.
APA Zhou, Xiaoting.,Gao, Yan.,Wang, Weiwei.,Yang, Xiaolin.,Yang, Xiuna.,...&Gong, Hongri.(2021).Architecture of the mycobacterial succinate dehydrogenase with a membrane-embedded Rieske FeS cluster.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,118.
MLA Zhou, Xiaoting,et al."Architecture of the mycobacterial succinate dehydrogenase with a membrane-embedded Rieske FeS cluster".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 118(2021).

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来源：合肥物质科学研究院

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