Multistory Stairs-based, Fast and Point-of-care Testing for Disease Biomarker Using One-step Capillary Microfluidic Fluoroimmunoassay Chip via Continuous On-chip Labelling
文献类型：期刊论文
| 作者 | Gao, Nailong2,3; Chang, Jianguo3; Zhu, Ziming1; You, Hui1
|
| 刊名 | BIOCHIP JOURNAL
 |
| 出版日期 | 2021-05-27 |
| 关键词 | Microfluidic Immunoassay microchip Delay valve On-chip labelling POCT |
| ISSN号 | 1976-0280 |
| DOI | 10.1007/s13206-021-00025-0 |
| 通讯作者 | You, Hui(usmlhy@iim.ac.cn) |
| 英文摘要 | Sufficiently long reaction time and low flow velocity are crucial for capillary microfluidic immunoassay chips, especially for truly "one-step" detection with continuous on-chip labelling and capturing. In this study, a multistory stairs-based new delay valve is used to meet the time and flow requirements for protein testing. The delay strategy is capable of slowing down the flow of liquid into the functional region and delaying the time out of the regions as well, thus extending the labelling and capture time to about 100 and 60 s respectively, and further reducing the flow velocity to below 8 mu L/min. Based on the principle of sandwich-like immunocomplex, the designed chip is capable of automatically accomplishing on-chip mixing, labelling and capturing procedures via once sample loading and sensitively detecting human Alpha-Fetoprotein (AFP) in 40 mu L serum with a wide dynamic ranging from 10 ng/mL to 10 000 ng/mL within 5 min. It could be foreseeable that this microchip has great potential in biomarkers clinical testing. |
| WOS关键词 | IMMUNOASSAY ; PROTEINS |
| 资助项目 | Key Research and Development Plan Project of Anhui Province[1704a0802157] ; Key-Area Research and Development Program of Guangdong Province[2019B020219003] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000655716000001 |
| 出版者 | KOREAN BIOCHIP SOCIETY-KBCS |
| 资助机构 | Key Research and Development Plan Project of Anhui Province ; Key-Area Research and Development Program of Guangdong Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/122620]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | You, Hui |
| 作者单位 | 1.Guangxi Univ, Sch Mech Engn, Nanning 530004, Guangxi, Peoples R China 2.Univ Sci & Technol China, Dept Precis Machinery & Precis Instrumentat, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Nailong,Chang, Jianguo,Zhu, Ziming,et al. Multistory Stairs-based, Fast and Point-of-care Testing for Disease Biomarker Using One-step Capillary Microfluidic Fluoroimmunoassay Chip via Continuous On-chip Labelling[J]. BIOCHIP JOURNAL,2021. |
| APA | Gao, Nailong,Chang, Jianguo,Zhu, Ziming,&You, Hui.(2021).Multistory Stairs-based, Fast and Point-of-care Testing for Disease Biomarker Using One-step Capillary Microfluidic Fluoroimmunoassay Chip via Continuous On-chip Labelling.BIOCHIP JOURNAL. |
| MLA | Gao, Nailong,et al."Multistory Stairs-based, Fast and Point-of-care Testing for Disease Biomarker Using One-step Capillary Microfluidic Fluoroimmunoassay Chip via Continuous On-chip Labelling".BIOCHIP JOURNAL (2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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