Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3 beta-Mediated Pathways
文献类型：期刊论文
| 作者 | Yu, Wen-Ying3; Cai, Wei2; Ying, Hua-Zhong3; Zhang, Wen-You3; Zhang, Huan-Huan3; Yu, Chen-Huan1,3,4 |
| 刊名 | JOURNAL OF INFLAMMATION RESEARCH
 |
| 出版日期 | 2021 |
| 卷号 | 14 |
| 关键词 | cross-kingdom regulation miRNA library GSK-3 beta apoptosis hepatic fibrosis inflammation |
| DOI | 10.2147/JIR.S304828 |
| 通讯作者 | Yu, Chen-Huan(yuchenhuan2002@163.com) |
| 英文摘要 | Purpose: Plant-derived exogenous microRNAs (miRNAs) regulate human physiological functions by blocking the translation of target mRNAs. Although several computational approaches have been developed to elucidate the interactions of cross-species miRNAs and their targets in mammals, the number of verified plant miRNAs is still limited, and the biological roles of most exogenous plant miRNAs remain unknown. Methods: A miRNA mimic library-based phenotypic screening, which contained 8394 plant mature miRNAs published in the official database miRbase, was performed to identify more novel bioactive plant miRNAs for the prevention of hepatic fibrosis. Inhibition of candidates for the activation of hepatic stellate cells (HSCs) and the underlying mechanisms were evaluated in TGF-beta 1- and PDGF-exposed HSC models. The protective effects of the candidates against CCl4-induced liver fibrosis were evaluated in a mouse model. Results: Among the 8394 plant mature miRNAs reported in the official database miRBase, five candidates were found to effectively inhibit the differentiation of HSCs. gma-miR-159a (miR159a) exerted the strongest inhibitory activities on both TGF-beta 1- and PDGF-induced HSC activation and proliferation by inhibiting the GSK-3 beta-mediated NF-kappa B and TGF-beta 1 pathways. Moreover, miR159a was mainly accumulated in the liver after intravenous injection, and it reduced CCl4-induced hepatic fibrosis and inflammation in mice. Conclusion: Results indicated that miR159a has the therapeutic potential for preventing hepatic fibrosis. This study provides a novel strategy for achieving natural nucleic acid drugs. |
| WOS关键词 | FIBROSIS ; MICRORNAS ; CANCER ; GSK-3 |
| 资助项目 | Natural Science Foundation of Zhejiang Province[LZ17H280001] ; National Natural Science Foundation of China[81473339] ; National Natural Science Foundation of China[81573591] ; National Natural Science Foundation of Ningbo[2015A610282] ; Zhejiang Medical science and tech-nology program[2015ZB104] ; Zhejiang Medical science and tech-nology program[2015KYA065] |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000653394600001 |
| 出版者 | DOVE MEDICAL PRESS LTD |
| 资助机构 | Natural Science Foundation of Zhejiang Province ; National Natural Science Foundation of China ; National Natural Science Foundation of Ningbo ; Zhejiang Medical science and tech-nology program |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/122671]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Yu, Chen-Huan |
| 作者单位 | 1.Chinese Acad Sci, Inst Canc & Basic Med, Dongfang St 150, Hangzhou 310018, Zhejiang, Peoples R China 2.Zhejiang Pharmaceut Coll, Dept Tradit Chinese Med, Ningbo, Peoples R China 3.Hangzhou Med Coll, Zhejiang Acad Med Sci, Zhejiang Key Lab Expt Anim & Safety Evaluat, Hangzhou, Peoples R China 4.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Hangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Wen-Ying,Cai, Wei,Ying, Hua-Zhong,et al. Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3 beta-Mediated Pathways[J]. JOURNAL OF INFLAMMATION RESEARCH,2021,14. |
| APA | Yu, Wen-Ying,Cai, Wei,Ying, Hua-Zhong,Zhang, Wen-You,Zhang, Huan-Huan,&Yu, Chen-Huan.(2021).Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3 beta-Mediated Pathways.JOURNAL OF INFLAMMATION RESEARCH,14. |
| MLA | Yu, Wen-Ying,et al."Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3 beta-Mediated Pathways".JOURNAL OF INFLAMMATION RESEARCH 14(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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