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G-Quadruplex-Induced Liquid-Liquid Phase Separation in Biomimetic Protocells

文献类型：期刊论文

作者Liu, Xuejiao7; Xiong, Yansong2; Zhang, Chunjuan7; Lai, Rongji7; Liu, Hui7; Peng, Ruizi1,7; Fu, Ting1; Liu, Qiaoling7; Fang, Xiaohong1,2; Mann, Stephen3,4,5,6
刊名JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
出版日期2021-07-28
卷号143
ISSN号0002-7863
DOI10.1021/jacs.1c03627
通讯作者Liu, Qiaoling(qlliu@iccas.ac.cn) ; Fang, Xiaohong(xfang@iccas.ac.cn) ; Mann, Stephen(s.mann@bristol.ac.uk)
英文摘要Biomolecular condensates comprised of specific proteins and nucleic acids are now recognized as one of the key organizing mechanisms in eukaryotic cells. However, the specific roles played by the nucleic acid secondary structure and sequence in biomolecular phase separation are still not clear. Here, utilizing giant membrane vesicles (GMVs) as a protocell model, we found that single-stranded DNA (ssDNA) with a parallel G-quadruplex structure could functionally cooperate with a G-quadruplex-binding protein to form speckle-like puncta inside the GMVs. The clustering behavior is dependent on the structural diversity of G-quadruplexes, and the reversible clustering behavior implicated a new pathway in dynamically regulating the formation of biomolecular condensates. This finding represents a potential link between G-quadruplexbinding proteins and the resulting G-quadruplex-mediated biomolecular phase separation, which would gain insight into a wide range of biological processes associated with nucleic acid-modulated phase separation inside living cells.
WOS关键词RNA ; DNA ; STRESS ; SERBP1 ; PHOSPHORYLATION ; TRANSITION ; RECEPTOR ; BIOLOGY ; MODEL ; FUS
资助项目NSFC[22022701] ; NSFC[21735006] ; NSFC[91939301] ; NSFC[21827811] ; NSFC[2019YFA0905800] ; NSFC[21991084] ; Hunan Provincial Key Area RD Program[2019SK2201] ; Beijing National Laboratory for Molecular Sciences[BNLMS201806] ; Department of Chinese Academy of Sciences Consultation and Evaluation Project[2019-C02-B-005]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000679913600030
出版者AMER CHEMICAL SOC
资助机构NSFC ; Hunan Provincial Key Area RD Program ; Beijing National Laboratory for Molecular Sciences ; Department of Chinese Academy of Sciences Consultation and Evaluation Project
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/123093]  
专题中国科学院合肥物质科学研究院
通讯作者Liu, Qiaoling; Fang, Xiaohong; Mann, Stephen
作者单位1.Chinese Acad Sci, Canc Hosp, Inst Basic Med & Canc IBMC, Univ Chinese Acad Sci,Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
3.Univ Bristol, Ctr Organized Matter Chem, Sch Chem, Bristol BS8 1TS, Avon, England
4.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Sch Mat Sci & Engn,Inst Mol Med IMM, Shanghai 200240, Peoples R China
5.Univ Bristol, Max Planck Bristol Ctr Minimal Biol, Sch Chem, Bristol BS8 1TS, Avon, England
6.Univ Bristol, Ctr Protolife Res, Bristol BS8 1TS, Avon, England
7.Hunan Univ, Mol Sci & Biomed Lab MBL, State Key Lab Chemo Biosensing & Chemometr, Coll Biol,Coll Chem & Chem Engn Aptamer Engn Ctr, Changsha 410082, Hunan, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Xuejiao,Xiong, Yansong,Zhang, Chunjuan,et al. G-Quadruplex-Induced Liquid-Liquid Phase Separation in Biomimetic Protocells[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2021,143.
APA Liu, Xuejiao.,Xiong, Yansong.,Zhang, Chunjuan.,Lai, Rongji.,Liu, Hui.,...&Tan, Weihong.(2021).G-Quadruplex-Induced Liquid-Liquid Phase Separation in Biomimetic Protocells.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,143.
MLA Liu, Xuejiao,et al."G-Quadruplex-Induced Liquid-Liquid Phase Separation in Biomimetic Protocells".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 143(2021).

入库方式： OAI收割

来源：合肥物质科学研究院

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