In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae
文献类型：期刊论文
| 作者 | Liu, Jia2; Yang, Chunjun2; Cheng, Cheng1; Zhang, Chenchen2; Zhao, Jun2; Fu, Chuyu2 |
| 刊名 | BIOENGINEERED
 |
| 出版日期 | 2021 |
| 卷号 | 12 |
| 关键词 | Neisseria gonorrhoeae plasma-activated liquid biocidal efficacy antimicrobial mechanism inflammation |
| ISSN号 | 2165-5979 |
| DOI | 10.1080/21655979.2021.1955548 |
| 通讯作者 | Yang, Chunjun(yangchunjun9@163.com) |
| 英文摘要 | Neisseria gonorrhoeae (Ng) is highly resistant to treatment, and there is an urgent need for new treatments to alleviate gonococcal resistance caused by antibiotic monotherapy. The antimicrobial effect and mechanism of plasma-activated liquid (PAL) on Ng were evaluated in this study. Upon PAL treatment, extensively analyses on cell culturability, metabolic capacity, intracellular reactive oxygen species (ROS),membrane integrity and nucleic acids for Ng were carried out and significant antimicrobial effects observed.PAL exerted antibacterial effect on Ng and induced bacterial death (6.71-log) following immersion for 30 min and treatment for 120 s. However, bacterial viability test revealed that after immersion in the same PAL, 10.17% of bacteria retained their metabolic capacity. This indicates that bacteria enter a physiological viable but non-culturable state to protect themselves from environmental stress. Confocal fluorescence microscopy and transmission electron microscopy demonstrated that PAL exerts bactericidal effect on Ng and disrupts its morphological structure. PAL may upregulate inflammatory factors and genes to modulate the resistance of Ng and affect the immune status of the host during infection. |
| WOS关键词 | ESCHERICHIA-COLI ; STAPHYLOCOCCUS-AUREUS ; SPOILAGE CAPABILITY ; LIPID-PEROXIDATION ; INACTIVATION ; RESISTANCE ; NLRP3 ; STATE ; WATER |
| 资助项目 | Clinical Research Cultivation Program of The Second Affiliated Hospital of Anhui Medical University[2020LCZD21] ; Natural Science Research Project of Anhui Universities[KJ2020ZD19] ; National Natural Science Foundation of China[51777206] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000679091200001 |
| 出版者 | TAYLOR & FRANCIS INC |
| 资助机构 | Clinical Research Cultivation Program of The Second Affiliated Hospital of Anhui Medical University ; Natural Science Research Project of Anhui Universities ; National Natural Science Foundation of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/123149]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Yang, Chunjun |
| 作者单位 | 1.Chinese Acad Sci, Inst Plasma Phys, Hefei, Peoples R China 2.Anhui Med Univ, Affiliated Hosp 2, Dept Dermatol, Hefei 230601, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jia,Yang, Chunjun,Cheng, Cheng,et al. In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae[J]. BIOENGINEERED,2021,12. |
| APA | Liu, Jia,Yang, Chunjun,Cheng, Cheng,Zhang, Chenchen,Zhao, Jun,&Fu, Chuyu.(2021).In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae.BIOENGINEERED,12. |
| MLA | Liu, Jia,et al."In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae".BIOENGINEERED 12(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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