METTL3-induced UCK2 m(6)A hypermethylation promotes melanoma cancer cell metastasis via the WNT/beta-catenin pathway
文献类型：期刊论文
| 作者 | Wu, Hao1,2 ; Xu, Haochao1,2; Jia, Dongdong1,2; Li, Tao1,2; Xia, Liming1,2
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| 刊名 | ANNALS OF TRANSLATIONAL MEDICINE
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| 出版日期 | 2021-07-13 |
| ISSN号 | 2305-5839 |
| 关键词 | Uridine-cytidine kinase 2 (UCK2) database m(6)A metastasis melanoma cancer |
| DOI | 10.21037/atm-21-2906 |
| 通讯作者 | Xia, Liming(doctorxlm@sina.com) |
| 英文摘要 | Background: Melanoma is a highly aggressive, malignant skin tumor with a statistically high mortality rate. N6-methyladenosine (m(6)A) modification is involved in a variety of biological processes, including tumorigenesis. m(6)A modifications regulate the fate and functions of RNA, such as mRNA stability, nuclear processing, transport, localization, translation, primary microRNA (miRNA) processing, and RNA-protein interactions. Several members (including METTL3, METTL14, FTO, ALKBH5, and YTHDF2) are actively involved in a variety of human cancers. However, the basic mechanism of the involvement of uridine cytidine kinase 2 (UCK2) in melanoma metastasis has not been studied. UCK2 is upregulated in a variety of malignancies. However, the complex molecular mechanisms and therapeutic effects of UCK2 in melanoma remain unclear. Methods: The expression of UCK2 was evaluated by qRT-PCR. The effects of UCK2 on the biological characteristics of PC cells were investigated on the basis of loss-of-function analyses. ImmunoprecipitationqPCR (MeRIP-qPCR) was performed to identify the m(6)A targeted effect of UCK2 in melanoma cancer. Results: Based on the bioinformatics analysis in this study, up-regulation of UCK2 could be essential in melanoma cancer, and associated with poor survival. Furthermore, the m(6)A modification regulated by METTL3 led to UCK2 increased messenger RNA (mRNA) stability in melanoma cancer. Functional and mechanistic experiments indicated that UCK2 enhanced the metastasis of melanoma cancer cells through the WNT/13-catenin pathway. Conclusion: In this study, we found that m(6)A-METTL3 axis induced abnormal UCK2 expression plays a role in melanoma metastasis by enhancing the Wnt/13-catenin pathway, which may provide new clues for melanoma metastasis. It also provides a potential target for the prevention and treatment of melanoma. |
| WOS关键词 | CYTIDINE KINASE 2 ; PROLIFERATION ; STATISTICS ; ACTIVATION ; EXPRESSION ; BIOMARKER ; INVASION ; ASSAY |
| 资助项目 | Health Science and Technology program of Zhejiang Province[2021KY106] |
| WOS研究方向 | Oncology ; Research & Experimental Medicine |
| 语种 | 英语 |
| 出版者 | AME PUBL CO |
| WOS记录号 | WOS:000677657200001 |
| 资助机构 | Health Science and Technology program of Zhejiang Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/123200]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Xia, Liming |
| 作者单位 | 1.Univ Chinese Acad Sci, Canc Hosp, Dept Bone & Soft Tissue Surg, Zhejiang Canc Hosp, Hangzhou, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Hao,Xu, Haochao,Jia, Dongdong,et al. METTL3-induced UCK2 m(6)A hypermethylation promotes melanoma cancer cell metastasis via the WNT/beta-catenin pathway[J]. ANNALS OF TRANSLATIONAL MEDICINE,2021. |
| APA | Wu, Hao,Xu, Haochao,Jia, Dongdong,Li, Tao,&Xia, Liming.(2021).METTL3-induced UCK2 m(6)A hypermethylation promotes melanoma cancer cell metastasis via the WNT/beta-catenin pathway.ANNALS OF TRANSLATIONAL MEDICINE. |
| MLA | Wu, Hao,et al."METTL3-induced UCK2 m(6)A hypermethylation promotes melanoma cancer cell metastasis via the WNT/beta-catenin pathway".ANNALS OF TRANSLATIONAL MEDICINE (2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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