Exosomes Derived from Nerve Stem Cells Loaded with FTY720 Promote the Recovery after Spinal Cord Injury in Rats by PTEN/AKT Signal Pathway
文献类型：期刊论文
| 作者 | Chen, Jianbin2; Zhang, Can3; Li, Shouye4; Li, Zheming4; Lai, Xiaojing1,5; Xia, Qingqing6 |
| 刊名 | JOURNAL OF IMMUNOLOGY RESEARCH
 |
| 出版日期 | 2021-07-14 |
| 卷号 | 2021 |
| ISSN号 | 2314-8861 |
| DOI | 10.1155/2021/8100298 |
| 通讯作者 | Lai, Xiaojing(czz20160127@163.com) ; Xia, Qingqing(418714257@qq.com) |
| 英文摘要 | Background. Spinal cord injury (SCI) remains a challenge owing to limited therapies. The exosome of neural stem cells (NSCs-Exos) and FTY720 transplantation could improve SCI effectively. However, the effect and mechanism of NSCs-Exos combined with FTY720 (FTY720-NSCs-Exos) transplantation in the treatment of SCI are not fully understood. Methods. Sprague Dawley rats (8-week-old) were used to establish the SCI model, followed by the treatment of NSCs-Exos, FTY720, and FTY720-NSCs-Exos. The effect of FTY720, NSCs-Exos, and FTY720-NSCs-Exos combination treatment on hindlimb function, pathological changes, apoptosis activity, and the expression of spinal edema-related proteins and apoptosis-related proteins in SCI models were investigated by BBB scoring, HE staining, TUNEL staining and immunohistochemistry, and Western blotting. Meanwhile, the effect of these treatments on spinal cord microvascular endothelial cells (SCMECs) was detected under hypoxic circumstance. Results. Our results found that FTY720-NSCs-Exos could alleviate pathological alterations and ameliorate the hindlimb function and oxygen insufficiency in model mice after SCI. In addition, exosomes could ameliorate the morphology of neurons, reduce inflammatory infiltration and edema, decrease the expression of Bax and AQP-4, upregulate the expression of claudin-5 and Bcl-2, and inhibit cell apoptosis. At the same time, in vitro experiments showed that FTY720-NSCs-Exos could protect the barrier of SCMECs under hypoxic circumstance, and the mechanism is related to PTEN/AKT pathway. Conclusion. FTY720-NSCs-Exos therapy displayed a positive therapeutic effect on SCI by regulating PTEN/AKT pathway and offered a new therapy for SCI. |
| WOS关键词 | MICROVASCULAR PERICYTES ; BRAIN ; EXPRESSION ; NEURONS ; EDEMA |
| 资助项目 | Basic Public Welfare Research Project of Zhejiang Province[LGF19H060001] |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000680840700004 |
| 出版者 | HINDAWI LTD |
| 资助机构 | Basic Public Welfare Research Project of Zhejiang Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/123212]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Lai, Xiaojing; Xia, Qingqing |
| 作者单位 | 1.Chinese Acad Sci, Inst Basic Med & Canc IBMC, Banshan East Rd 1, Hangzhou 310022, Zhejiang, Peoples R China 2.Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Neurosurg, Wuhan 430030, Hubei, Peoples R China 3.Hunan Univ, Coll Biol, Dept Biomed Engn, Changsha 410082, Hunan, Peoples R China 4.Hangzhou Med Coll, Coll Pharm, Hangzhou 310022, Zhejiang, Peoples R China 5.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 6.Wenzhou Med Univ, Taizhou Peoples Hosp 1, Huangyan Hosp, Dept Lab Med, Hengjie Rd 218, Taizhou 318020, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jianbin,Zhang, Can,Li, Shouye,et al. Exosomes Derived from Nerve Stem Cells Loaded with FTY720 Promote the Recovery after Spinal Cord Injury in Rats by PTEN/AKT Signal Pathway[J]. JOURNAL OF IMMUNOLOGY RESEARCH,2021,2021. |
| APA | Chen, Jianbin,Zhang, Can,Li, Shouye,Li, Zheming,Lai, Xiaojing,&Xia, Qingqing.(2021).Exosomes Derived from Nerve Stem Cells Loaded with FTY720 Promote the Recovery after Spinal Cord Injury in Rats by PTEN/AKT Signal Pathway.JOURNAL OF IMMUNOLOGY RESEARCH,2021. |
| MLA | Chen, Jianbin,et al."Exosomes Derived from Nerve Stem Cells Loaded with FTY720 Promote the Recovery after Spinal Cord Injury in Rats by PTEN/AKT Signal Pathway".JOURNAL OF IMMUNOLOGY RESEARCH 2021(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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