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High Expression Levels of CDK1 and CDC20 in Patients With Lung Squamous Cell Carcinoma are Associated With Worse Prognosis

文献类型：期刊论文

作者Deng, Huan2,3,4,5; Hang, Qingqing6; Shen, Dijian2,3,4; Ying, Hangjie2,3,4; Zhang, Yibi1; Qian, Xu2,3,4; Chen, Ming2,3,4
刊名FRONTIERS IN MOLECULAR BIOSCIENCES
出版日期2021-07-07
卷号8
关键词lung squamous cell carcinoma protein-protein interaction network survival analysis gene set enrichment analysis experimental validation
DOI10.3389/fmolb.2021.653805
通讯作者Qian, Xu(Qian_michelle2014@163.com) ; Chen, Ming(chenming@zjcc.org.cn)
英文摘要Purpose: Progress related to the early detection and molecular targeted therapy of lung squamous cell carcinoma (LUSC) remains limited. The goal of our study was to identify key candidate indicators of LUSC. Methods: Three microarray datasets (GSE33532, GSE30219 and GSE19188) were applied to find differentially expressed genes (DEGs). Functional enrichment analyses of DEGs were carried out, and their protein-protein interaction (PPI) network was established. Hub genes were chosen from the PPI network according to their degree scores. Then, overall survival (OS) analyses of hub genes were carried out using Kaplan-Meier plotter, and their GSEA analyses were performed. Public databases were used to verify the expression patterns of CDK1 and CDC20. Furthermore, basic experiments were performed to verify our findings. Results: A total of 1,366 DEGs were identified, containing 669 downregulated and 697 upregulated DEGs. These DEGs were primarily enriched in cell cycle, chromosome centromeric region and nuclear division. Seventeen hub genes were selected from PPI network. Survival analyses demonstrated that CDK1 and CDC20 were closely associated with OS. GSEA analyses revealed that cell cycle, DNA replication, and mismatch repair were associated with CDK1 expression, while spliceosome, RNA degradation and cell cycle were correlated with CDC20 expression. Based on The Cancer Genome Atlas (TCGA) and The Human Protein Atlas (THPA) databases, CDK1 and CDC20 were upregulated in LUSC at the mRNA and protein levels. Moreover, basic experiments also supported the obvious upregulation of CDK1 and CDC20 in LUSC. Conclusion: Our study suggests and validates that CDK1 and CDC20 are potential therapeutic targets and prognostic biomarkers of LUSC.
WOS关键词CANCER ; OVEREXPRESSION ; PROMOTES
资助项目National Natural Science Foundation of China[81672972] ; Zhejiang Provincial Key Research and Development Program[2019C03003]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000674915400001
出版者FRONTIERS MEDIA SA
资助机构National Natural Science Foundation of China ; Zhejiang Provincial Key Research and Development Program
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/123327]  
专题中国科学院合肥物质科学研究院
通讯作者Qian, Xu; Chen, Ming
作者单位1.Nanchang Univ, Jiangxi Med Coll, Nanchang, Jiangxi, Peoples R China
2.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Hangzhou, Peoples R China
3.Chinese Acad Sci, Inst Canc Res & Basic Med IBMC, Hangzhou, Peoples R China
4.Zhejiang Canc Hosp, Dept Radiat Oncol, Zhejiang Key Lab Radiat Oncol, Hangzhou, Peoples R China
5.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
6.Zhejiang Chinese Med Univ, Clin Med Coll 2, Hangzhou, Peoples R China
推荐引用方式
GB/T 7714		 Deng, Huan,Hang, Qingqing,Shen, Dijian,et al. High Expression Levels of CDK1 and CDC20 in Patients With Lung Squamous Cell Carcinoma are Associated With Worse Prognosis[J]. FRONTIERS IN MOLECULAR BIOSCIENCES,2021,8.
APA Deng, Huan.,Hang, Qingqing.,Shen, Dijian.,Ying, Hangjie.,Zhang, Yibi.,...&Chen, Ming.(2021).High Expression Levels of CDK1 and CDC20 in Patients With Lung Squamous Cell Carcinoma are Associated With Worse Prognosis.FRONTIERS IN MOLECULAR BIOSCIENCES,8.
MLA Deng, Huan,et al."High Expression Levels of CDK1 and CDC20 in Patients With Lung Squamous Cell Carcinoma are Associated With Worse Prognosis".FRONTIERS IN MOLECULAR BIOSCIENCES 8(2021).

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来源：合肥物质科学研究院

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