Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway
文献类型：期刊论文
| 作者 | Ni, Zhijian1,4; Wu, Jinyong2,3,4 ; Li, Zhongkui1,4; Yuan, Lixia4; Wang, Yu3; Chen, Xiangsong2,4; Yao, Jianming1,4
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| 刊名 | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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| 出版日期 | 2021-06-24 |
| 卷号 | 85 |
| 关键词 | Escherichia coli 3-fucosyllactose cofactors sorbitol fructose-6-phosphate |
| ISSN号 | 0916-8451 |
| DOI | 10.1093/bbb/zbab074 |
| 通讯作者 | Chen, Xiangsong(xschen@ipp.ac.cn) ; Yao, Jianming(jmyao@ipp.ac.cn) |
| 英文摘要 | 3-fucosyllactose (3-FL) and 2'-fucosyllactose (2'-FL), are two important fucosylated oligosaccharides in human milk. Extensive studies on 2'-FL enabled its official approval for use in infant formula. However, development of 3-FL has been somewhat sluggish due to its low content in human milk and poor yield in enlarged production. Here, an alpha-1,3-fucosyltransferase mutant was introduced into an engineered Escherichia coli (E. coli) capable of producing GDP-L-fucose, leading to a promising 3-FL titer in a 5.0-L bioreactor. To increase the availability of cofactors (NADPH and GTP) for optimized 3-FL production, zwf, pntAB, and gsk genes were successively overexpressed, finally resulting in a higher 3-FL level with a titer of 35.72 g/L and a yield of 0.82 mol 3-FL/mol lactose. Unexpectedly, the deletion of pfkA gene led to a much lower performance of 3-FL production than the control strain. Still, our strategy achieved the highest 3-FL level in E. coli to date. [GRAPHICS] The metabolically engineered Escherichia coli effectively produced 3-fucosyllactose through the modified alpha-1,3-fucosyltransferase and the enhanced regeneration of cofactors (NADPH and GTP). |
| WOS关键词 | HUMAN-MILK OLIGOSACCHARIDES ; BIOTECHNOLOGICAL PRODUCTION ; HELICOBACTER-PYLORI ; FUTILE CYCLE ; ALPHA-1,3-FUCOSYL-TRANSFERASE ; 2'-FUCOSYLLACTOSE ; TRANSHYDROGENASE ; PCR |
| 资助项目 | Science and Technology Service Network Initiative of Chinese Academy of Science[KFJ-STS-QYZD-122] ; Science and Technology Planning Project of Wuhan City[2020020602012119] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000671178200026 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | Science and Technology Service Network Initiative of Chinese Academy of Science ; Science and Technology Planning Project of Wuhan City |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/123475]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Chen, Xiangsong; Yao, Jianming |
| 作者单位 | 1.Univ Sci & Technol China, Sci Isl Branch Grad Sch, Hefei, Peoples R China 2.Chinese Acad Sci, Huainan New Energy Res Ctr, Inst Plasma Phys, Huainan, Peoples R China 3.Wuhan Zhongke Opt Valley Green Biotechnol Co Ltd, Wuhan, Peoples R China 4.Chinese Acad Sci, Inst Plasma Phys, Hefei Inst Phys Sci, Hefei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ni, Zhijian,Wu, Jinyong,Li, Zhongkui,et al. Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2021,85. |
| APA | Ni, Zhijian.,Wu, Jinyong.,Li, Zhongkui.,Yuan, Lixia.,Wang, Yu.,...&Yao, Jianming.(2021).Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,85. |
| MLA | Ni, Zhijian,et al."Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 85(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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