Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging
文献类型：期刊论文
| 作者 | Fan Zi-Yan2; Liu Zheng-Jie2; Zhang Rui-Long2; Han Guang-Mei2; Zhang Zhong-Ping1,2
|
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2021-07-01 |
| 卷号 | 49 |
| ISSN号 | 0253-3820 |
| 关键词 | Carbon dots Functional modification N,N-dimethylethylenediamine Lysosome target Cell imaging |
| DOI | 10.1016/S1872-2040(21)60108-1 |
| 通讯作者 | Han Guang-Mei(gmhan@ahu.edu.cn) |
| 英文摘要 | The lysosome-targeting carbon dots (CDs) with excellent water solubility and strong blue emission were synthesized through a one-step functional modification for long-term lysosome imaging in living cells. Based on the carboxyl-rich CDs synthesized by oxidizing carbon black in refluxing HNO3, then N,N-dimethylethylenediamine (DMEDA) was chemically linked onto the CDs through acylation reaction to obtain DMEDA modified carbon dots (M-CDs). The infrared spectrum of M-CDs showed the characteristic peak of acylamide (O=C-NH-) at 1658 cm(-1), indicating the successful modification of DMEDA. The quantum yield of M-CDs was 15.6%, which was 27 times higher than the initial carboxyl rich CDs, meeting the requirement of cell imaging. The probe entered cells through the temperature-dependent endocytosis way and the co-localization experiment with commercial dye Lyso-Tracker red showed that the probe localized in lysosomes and could be used as a universal lysosome tracker in different cell lines. Moreover, compared to commercially available Lyso-Tracker blue, M-CDs were more photostable under UV light. The fluorescence of M-CDs was still brightness after time-lapsed imaging by confocal microscopy for 120 min with an interval of 5 min in living cells, implying that the M-CDs could be used for long-term imaging of lysosomes. |
| WOS关键词 | GRAPHENE QUANTUM DOTS ; FLUORESCENT-PROBE ; NANOPROBES |
| 资助项目 | National Natural Science Foundation of China[22074001] ; National Natural Science Foundation of China[21874137] ; National Natural Science Foundation of China[21974001] ; National Natural Science Foundation of China[21775001] ; Science and Technology Service Network Initiative of Chinese Academy of Sciences[KFJ-STS-ZDTP-080] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | SCIENCE PRESS |
| WOS记录号 | WOS:000670603700015 |
| 资助机构 | National Natural Science Foundation of China ; Science and Technology Service Network Initiative of Chinese Academy of Sciences |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/123493]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Han Guang-Mei |
| 作者单位 | 1.Chinese Acad Sci, Inst Solid State Phys, Key Lab Photovolta & Energy Conservat Mat, HFIPS, Hefei 230031, Peoples R China 2.Anhui Univ, Sch Chem & Chem Engn, Inst Phys Sci & Informat Technol, Informat Mat & Intelligent Sensing Lab Anhui Prov, Hefei 230601, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan Zi-Yan,Liu Zheng-Jie,Zhang Rui-Long,et al. Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2021,49. |
| APA | Fan Zi-Yan,Liu Zheng-Jie,Zhang Rui-Long,Han Guang-Mei,&Zhang Zhong-Ping.(2021).Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,49. |
| MLA | Fan Zi-Yan,et al."Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 49(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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