Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis
文献类型：期刊论文
| 作者 | Shao, Chang-Sheng3,4; Feng, Na1,2; Zhou, Shuai1; Zheng, Xin-Xin3,4; Wang, Peng4; Zhang, Jing-Song1,2; Huang, Qing3,4
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| 刊名 | TOXICOLOGY RESEARCH
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| 出版日期 | 2021-05-01 |
| 卷号 | 10 |
| 关键词 | ganoderic acid T (GAT) triterpene apoptosis necroptosis radiosensitivity ionizing radiation |
| ISSN号 | 2045-452X |
| DOI | 10.1093/toxres/tfab030 |
| 通讯作者 | Zhang, Jing-Song(syja16@saas.sh.cn) ; Huang, Qing(huangq@ipp.ac.cn) |
| 英文摘要 | The use of natural substances derived from traditional Chinese medicine and natural plants as safe radiosensitizing adjuvants is a new trend for cancer radiotherapy. Ganoderma lucidum has been used as a traditional Chinese medicine with a history of more than 2000 years. Ganoderic acid T (GAT) is a typical triterpene of G. lucidum, which has strong cytotoxicity to cancer cells, but whether it has radiation sensitization effect has not been explored. In this work, we treated the HeLa cells with different concentrations of GAT before exposure to gamma-ray radiation and investigated its influence on the radiosensitivity. The cell viability, apoptosis rate, necoptosis rate, intracellular ATP level, cell cycle, the amount of H2AX and 53BP1, reactive oxygen species, and mitochondrial membrane potential were examined. Apoptotic, necroptotic, and autophagic biomarker proteins, including caspase 8, cytochrome c, caspase 3, RIPK, MLKL, P62, and LC3, were analyzed. As a result, we confirmed that with treatment of GAT, the gamma-ray radiation induced both apoptosis and necroptosis in HeLa cells, and with increase of GAT, the percentage ratio of necroptosis was increased. The involved pathways and mechanisms were also explored and discussed. |
| WOS关键词 | CANCER-CELLS ; DEATH FATE ; INHIBITION ; LUCIDUM ; SWITCH |
| 资助项目 | National Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[11775272] ; Central-level scientific institutions[Y79XG13361] |
| WOS研究方向 | Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000671151100018 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of China ; Central-level scientific institutions |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/123505]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhang, Jing-Song; Huang, Qing |
| 作者单位 | 1.Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Beijing, Peoples R China 2.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Inst Edible Fungi, Shanghai 201403, Peoples R China 3.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shao, Chang-Sheng,Feng, Na,Zhou, Shuai,et al. Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis[J]. TOXICOLOGY RESEARCH,2021,10. |
| APA | Shao, Chang-Sheng.,Feng, Na.,Zhou, Shuai.,Zheng, Xin-Xin.,Wang, Peng.,...&Huang, Qing.(2021).Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis.TOXICOLOGY RESEARCH,10. |
| MLA | Shao, Chang-Sheng,et al."Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis".TOXICOLOGY RESEARCH 10(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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