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Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme

文献类型：期刊论文

作者Kang, Fuying1,2; Yu, Lu3; Xia, Yan1,2; Yu, Minling1,2; Xia, Lin4; Wang, Yuchuan4; Yang, Lin5,6; Wang, Tianyuan1; Gong, Weimin5,6; Tian, Changlin3,5,6
刊名ACS CATALYSIS
出版日期2021-05-07
卷号11
ISSN号2155-5435
关键词photosystem I rational design photosensitizer protein iron-sulfur (Fe4S4) cluster photocatalytic CO2 reductase
DOI10.1021/acscatal.1c00287
通讯作者Tian, Changlin(cltian@ustc.edu.cn) ; Liu, Xiaohong(liuxh@moon.ibp.ac.cn) ; Wang, Jiangyun(jwang@ibp.ac.cn)
英文摘要Photosystem I (PSI) is a very large membrane protein complex (similar to 1000 kDa) harboring P700*, the strongest reductant known in biological systems, which is responsible for driving NAD(P)(+) and ultimately for CO2 reduction. Although PSI is one of the most important components in the photosynthesis machinery, it has remained difficult to enhance PSI functions through genetic engineering due to its enormous complexity. Inspired by PSI's ability to undergo multiple-step photo-induced electron hopping from P700* to iron-sulfur [Fe4S4] clusters, we designed a 33 kDa miniature photocatalytic CO2-reducing enzyme (mPCE) harboring a chromophore (BpC) and two [Fe4S4] clusters (Fe-A/Fe-B). Through reduction potential fine-tuning, we optimized the multiple-step electron hopping from BpC to Fe-A/Fe-B, culminating in a CO2/HCOOH conversion quantum efficiency of 1.43%. As mPCE can be overexpressed with a high yield in Escherichia coli cells without requiring synthetic cofactors, further development along this route may result in rapid photo-enzyme quantum yield improvement and functional expansion through an efficient directed evolution process.
WOS关键词VINELANDII FE PROTEIN ; ELECTRON-TRANSFER ; REDUCTION POTENTIALS ; ENERGY-TRANSFER ; PHOTOSYSTEM-I ; REDOX STATE ; IRON ; CLUSTERS ; FERREDOXIN ; SEQUENCE
资助项目National Key Research and Development Program of China[2019YFA0904101] ; National Key Research and Development Program of China[2019YFA0405600] ; National Key Research and Development Program of China[2017YFA0503704] ; National Science Foundation of China[21750003] ; National Science Foundation of China[21890743] ; National Science Foundation of China[21837005] ; National Science Foundation of China[21825703] ; National Science Foundation of China[21927814] ; National Science Foundation of China[U1732275] ; Sanming Project of Medicine in Shenzhen[SZSM201811092] ; Youth Innovation Promotion Association CAS ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB37040000] ; Users with Excellence Program of Hefei Science Center, CAS[2019HSC-UE005]
WOS研究方向Chemistry
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000649106200062
资助机构National Key Research and Development Program of China ; National Science Foundation of China ; Sanming Project of Medicine in Shenzhen ; Youth Innovation Promotion Association CAS ; Strategic Priority Research Program of Chinese Academy of Sciences ; Users with Excellence Program of Hefei Science Center, CAS
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/123666]  
专题中国科学院合肥物质科学研究院
通讯作者Tian, Changlin; Liu, Xiaohong; Wang, Jiangyun
作者单位1.Chinese Acad Sci, Inst Biophys, Lab RNA Biol, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China
4.Chinese Acad Sci, Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China
5.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China
6.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
推荐引用方式
GB/T 7714		 Kang, Fuying,Yu, Lu,Xia, Yan,et al. Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme[J]. ACS CATALYSIS,2021,11.
APA Kang, Fuying.,Yu, Lu.,Xia, Yan.,Yu, Minling.,Xia, Lin.,...&Wang, Jiangyun.(2021).Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme.ACS CATALYSIS,11.
MLA Kang, Fuying,et al."Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme".ACS CATALYSIS 11(2021).

入库方式： OAI收割

来源：合肥物质科学研究院

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