NMR structural analysis of the yeast cytochrome c oxidase subunit Cox13 and its interaction with ATP
文献类型：期刊论文
| 作者 | Zhou, Shu1,2 ; Pettersson, Pontus2; Bjorck, Markus L.2; Dawitz, Hannah2; Brzezinski, Peter2; Maler, Lena2; adelroth, Pia2
|
| 刊名 | BMC BIOLOGY
 |
| 出版日期 | 2021-05-10 |
| 卷号 | 19 |
| 关键词 | ATP Membrane protein NMR Solution structure |
| DOI | 10.1186/s12915-021-01036-x |
| 通讯作者 | Maler, Lena(lena.maler@dbb.su.se) ; adelroth, Pia(pia.adelroth@dbb.su.se) |
| 英文摘要 | Background Mitochondrial respiration is organized in a series of enzyme complexes in turn forming dynamic supercomplexes. In Saccharomyces cerevisiae (baker's yeast), Cox13 (CoxVIa in mammals) is a conserved peripheral subunit of Complex IV (cytochrome c oxidase, CytcO), localized at the interface of dimeric bovine CytcO, which has been implicated in the regulation of the complex. Results Here, we report the solution NMR structure of Cox13, which forms a dimer in detergent micelles. Each Cox13 monomer has three short helices (SH), corresponding to disordered regions in X-ray or cryo-EM structures of homologous proteins. Dimer formation is mainly induced by hydrophobic interactions between the transmembrane (TM) helix of each monomer. Furthermore, an analysis of chemical shift changes upon addition of ATP revealed that ATP binds at a conserved region of the C terminus with considerable conformational flexibility. Conclusions Together with functional analysis of purified CytcO, we suggest that this ATP interaction is inhibitory of catalytic activity. Our results shed light on the structural flexibility of an important subunit of yeast CytcO and provide structure-based insight into how ATP could regulate mitochondrial respiration. |
| WOS关键词 | RESPIRATORY SUPERCOMPLEXES ; ASSIGNMENT ; BINDING ; SPECTROSCOPY ; PROTEINS ; COMPLEX ; SYSTEM ; RCF2 ; ARCHITECTURE ; RELAXATION |
| 资助项目 | Knut and Alice Wallenberg Foundation[2013.0006] ; Swedish Research Council (VR)[2018-03395] ; iNEXT - Horizon 2020 program of the European Commission[653706] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| 出版者 | BMC |
| WOS记录号 | WOS:000655036700002 |
| 资助机构 | Knut and Alice Wallenberg Foundation ; Swedish Research Council (VR) ; iNEXT - Horizon 2020 program of the European Commission |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/123681]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Maler, Lena; adelroth, Pia |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, High Field Magnet Lab, Hefei, Peoples R China 2.Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden |
| 推荐引用方式 GB/T 7714 | Zhou, Shu,Pettersson, Pontus,Bjorck, Markus L.,et al. NMR structural analysis of the yeast cytochrome c oxidase subunit Cox13 and its interaction with ATP[J]. BMC BIOLOGY,2021,19. |
| APA | Zhou, Shu.,Pettersson, Pontus.,Bjorck, Markus L..,Dawitz, Hannah.,Brzezinski, Peter.,...&adelroth, Pia.(2021).NMR structural analysis of the yeast cytochrome c oxidase subunit Cox13 and its interaction with ATP.BMC BIOLOGY,19. |
| MLA | Zhou, Shu,et al."NMR structural analysis of the yeast cytochrome c oxidase subunit Cox13 and its interaction with ATP".BMC BIOLOGY 19(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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