Simultaneous detection of tumor-related mRNA and miRNA in cancer cells with magnetic SERS nanotags
文献类型：期刊论文
| 作者 | Li, Man4; Li, Jingya2,3; Zhang, Xiang4; Yao, Mingming4; Li, Pan1; Xu, Weiping4,5 |
| 刊名 | TALANTA
 |
| 出版日期 | 2021-09-01 |
| 卷号 | 232 |
| 关键词 | p21 mRNA microRNA-21 Duplex-specific nuclease signal amplification (DSNSA) Simultaneous detection The SERS-Based platform |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2021.122432 |
| 通讯作者 | Li, Pan(lipan2011@iim.ac.cn) ; Xu, Weiping(weipingx@ustc.edu.cn) |
| 英文摘要 | The design of simultaneous detection method has broad prospects for cancer diagnosis and prognosis. Herein, we reported a low cost and sensitive SERS sensing platform for simultaneous p21 mRNA and miRNA-21 detection based on duplex-specific nuclease signal amplification (DSNSA) plus multifunctional Fe3O4@SiO2 magnetic nanoparticles (Fe3O4@SiO2 MNPs). Here, Fe3O4@SiO2 MNPs were used as a separation substrate, and Au@AgNPs served as stable and ultrasensitive SERS nanotags. Firstly, Au@AgNPs and Fe3O4@SiO2 MNPs were attached to both ends of capture probe (CP) by covalent bonds. Under the assistance of the target p21 mRNA and miRNA-21, DNA (CP) of the DNA-RNA heteroduplexes could be specifically degraded by DSN and the SERS nanotags that were released from the surface of Fe3O4@SiO2 MNPs. Meanwhile, the target p21 mRNA and miRNA-21 were released and then involved in the next round of signal reactions. The proposed strategy allowed quantitative detection of p21 mRNA and miRNA-21 and the limit of detection (LOD) was 0.12 fM and 0.17 fM, respectively. This method gives a great potential for multiplex detection of biological molecules. |
| WOS关键词 | DUPLEX-SPECIFIC NUCLEASE ; SIGNAL AMPLIFICATION STRATEGY ; MICRORNAS ; CORE ; NANOPARTICLES ; EXPRESSION ; MIR-21 ; SHELL |
| 资助项目 | National Natural Science Foundation of China[51672004] ; Natural Science Foundation of Anhui Province (China)[1608085MH176] ; Natural Science Foundation of Anhui Province (China)[1908085QB65] ; Key Research and Development Program of Anhui Province (China)[1804h08020252] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000658317000039 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province (China) ; Key Research and Development Program of Anhui Province (China) |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/123744]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Li, Pan; Xu, Weiping |
| 作者单位 | 1.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Hefei 230021, Peoples R China 2.Univ Sci & Technol China, Dept Biol Phys, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China 4.Univ Sci & Technol China, Affiliated Hosp USTC 1, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China 5.Anhui Prov Key Lab Tumor Immunotherapy & Nutr The, Hefei 230001, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Man,Li, Jingya,Zhang, Xiang,et al. Simultaneous detection of tumor-related mRNA and miRNA in cancer cells with magnetic SERS nanotags[J]. TALANTA,2021,232. |
| APA | Li, Man,Li, Jingya,Zhang, Xiang,Yao, Mingming,Li, Pan,&Xu, Weiping.(2021).Simultaneous detection of tumor-related mRNA and miRNA in cancer cells with magnetic SERS nanotags.TALANTA,232. |
| MLA | Li, Man,et al."Simultaneous detection of tumor-related mRNA and miRNA in cancer cells with magnetic SERS nanotags".TALANTA 232(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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