A two-photon "turn-on" fluorescent probe for both exogenous and endogenous selenocysteine detection and imaging in living cells and zebrafish
文献类型：期刊论文
| 作者 | Zhao, Mei1; Shi, Di1; Hu, Wandi1; Ma, Tao4; He, Lei2; Lu, Danqing1; Hu, Yunchu1; Zhou, Liyi3 |
| 刊名 | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
 |
| 出版日期 | 2021-11-05 |
| 卷号 | 260 |
| 关键词 | Endogenous selenocysteine Two-photon fluorescence imaging Two-photon fluorescent probe Cell imaging Zebrafish |
| ISSN号 | 1386-1425 |
| DOI | 10.1016/j.saa.2021.119983 |
| 通讯作者 | Lu, Danqing(danqinglu@csuft.edu.cn) ; Hu, Yunchu(hucsfu@163.com) ; Zhou, Liyi(zhouly0817@163.com) |
| 英文摘要 | Selenocysteine (Sec) is recognized as the 21st amino acid employing as an essential building block for selenoproteins (SePs), which plays a significant role in various physiological processes. Therefore, there is an urgent need to reasonably develop some reliable and rapid methods for Sec detection in biological systems. In this work, we reported a new two-photon (TP) fluorescent probe BNT-Sec for Sec detection and imaging in living cells and zebrafish with two part: (1) a D-p-A-structured naphthalene derivative as a TP fluorophore; (2) a well-know Sec responsive site with strong intromolecular charge transfer effect (ICT) to selectively detect endogenous and exogenous. In the presence of Sec, probe BNT-Sec can initiate a Se-dependent specific aromatic nucleophilic substitution reaction, which exhibited BNT-Sec had a large fluorescence intensity enhancement with-18.9-fold at 510 nm, a high sensitivity low LOD value' 10.6 nM, good light stability, strong specificity, pH stability and low cytotoxicity. In addition, BNT-Sec can be conveniently used to detect Sec in living cells and zebrafish for TP imaging due to the great TP measurement properties of fluorophore, exhibiting it has the potential to reveal the role of selenocysteine in physiological and pathological processes in further biological applications. (C) 2021 Elsevier B.V. All rights reserved. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; SELENIUM ; SELENOPROTEINS ; SELENOMETHIONINE ; IDENTIFICATION ; SPECIATION ; NANOPROBE ; HEALTH ; CANCER |
| 资助项目 | National Natural Science Foundation for Yong Scientists of China[22004132] ; Natural Science Foundation of Hunan Province[2020JJ5963] ; Natural Science Foundation of Hunan Province[2020JJ4940] ; Natural Science Foundation of Hunan Province[2018JJ2671] ; National Postdoctoral Program of Innovative Talents of China[BX20200302] ; Research Foundation of Education Bureau of Hunan Province[19C1892] ; Research Foundation of Education Bureau of Hunan Province[18C0248] ; Undergraduate Training Program for Innovation & Entrepreneurship of Hunan Province[1624 [2019]] ; Science and Technology Project of Changsha, Hunan Province[kq1901140] ; Scientific Research Start-up Project of Central South University of Forestry and Technology[2017YJ02] |
| WOS研究方向 | Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000661321300019 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | National Natural Science Foundation for Yong Scientists of China ; Natural Science Foundation of Hunan Province ; National Postdoctoral Program of Innovative Talents of China ; Research Foundation of Education Bureau of Hunan Province ; Undergraduate Training Program for Innovation & Entrepreneurship of Hunan Province ; Science and Technology Project of Changsha, Hunan Province ; Scientific Research Start-up Project of Central South University of Forestry and Technology |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/123856]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Lu, Danqing; Hu, Yunchu; Zhou, Liyi |
| 作者单位 | 1.Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China 2.Univ Chinese Acad Sci, Chinese Acad Sci, Canc Hosp, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China 3.Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Deep Proc Rice & Byprod, Changsha 410004, Hunan, Peoples R China 4.Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Mei,Shi, Di,Hu, Wandi,et al. A two-photon "turn-on" fluorescent probe for both exogenous and endogenous selenocysteine detection and imaging in living cells and zebrafish[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2021,260. |
| APA | Zhao, Mei.,Shi, Di.,Hu, Wandi.,Ma, Tao.,He, Lei.,...&Zhou, Liyi.(2021).A two-photon "turn-on" fluorescent probe for both exogenous and endogenous selenocysteine detection and imaging in living cells and zebrafish.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,260. |
| MLA | Zhao, Mei,et al."A two-photon "turn-on" fluorescent probe for both exogenous and endogenous selenocysteine detection and imaging in living cells and zebrafish".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 260(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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