NLRP6 self-assembles into a linear molecular platform following LPS binding and ATP stimulation
文献类型：期刊论文
| 作者 | Leng, Fangwei6; Yin, Hang4; Qin, Siying4; Zhang, Kai6; Guan, Yukun4; Fang, Run4; Wang, Honglei1; Li, Guohui1; Jiang, Zhengfan4; Sun, Fei6 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2020 |
| 卷号 | 10 |
| ISSN号 | 2045-2322 |
| 其他题名 | NLRP6 self-assembles into a linear molecular platform following LPS binding and ATP stimulation |
| 英文摘要 | NOD-like receptors (NLRs) localize in the cytosol to recognize intracellular pathogen products and initialize the innate immune response. However, the ligands and ligand specificity of many NLRs remain unclear. One such NLR, NLRP6, plays an important role in maintaining intestinal homeostasis and protecting against various intestinal diseases such as colitis and intestinal tumorigenesis. Here, we show that the major component of the outer membrane of gram-negative bacteria, lipopolysaccharide (LPS), binds NLRP6 directly and induces global conformational change and dimerization. Following stimulation by ATP, the NLRP6 homodimer can further assemble into a linear molecular platform, and ASC is recruited to form higher molecular structures, indicative of a step-by-step activation mechanism. Our study sheds light on the mystery of LPS-induced inflammasome initiation, reveals the architecture and structural basis of potential pre-inflammasome, and suggests a novel molecular assembly pattern for immune receptors. |
| 资助项目 | [National Natural Science Foundation of China] ; [973 National Basic Research Plan of China] ; [Strategic Priority Research Program of the Chinese Academy of Sciences] ; [Chinese Academy of Sciences] |
| 语种 | 英语 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/124162]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 作者单位 | 1.Dalian Institute of Chemical Physics, Chinese Academy of Sciences 2.Purdue University System 3.Harvard University 4.Peking University 5.Hefei Institutes of Physical Science, Chinese Academy of Sciences 6.Institute of Biophysics, Chinese Academy of Sciences |
| 推荐引用方式 GB/T 7714 | Leng, Fangwei,Yin, Hang,Qin, Siying,et al. NLRP6 self-assembles into a linear molecular platform following LPS binding and ATP stimulation[J]. SCIENTIFIC REPORTS,2020,10. |
| APA | Leng, Fangwei.,Yin, Hang.,Qin, Siying.,Zhang, Kai.,Guan, Yukun.,...&Xie, Can.(2020).NLRP6 self-assembles into a linear molecular platform following LPS binding and ATP stimulation.SCIENTIFIC REPORTS,10. |
| MLA | Leng, Fangwei,et al."NLRP6 self-assembles into a linear molecular platform following LPS binding and ATP stimulation".SCIENTIFIC REPORTS 10(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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