Circular RNA circ_0020710 drives tumor progression and immune evasion by regulating the miR-370-3p
文献类型：期刊论文
| 作者 | Chuan-Yuan Wei2; Meng-Xuan Zhu2; Nan-Hang Lu2; Jia-Qi Liu2; Yan-Wen Yang2; Yong Zhang2 ; Yue-Dong Shi2; Zi-Hao Feng2; Jia-Xia Li1; Fa-Zhi Qi2
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| 刊名 | Molecular Cancer
 |
| 出版日期 | 2020 |
| 卷号 | 19 |
| ISSN号 | 1476-4598 |
| 其他题名 | Circular RNA circ_0020710 drives tumor progression and immune evasion by regulating the miR-370-3p |
| 英文摘要 | Background Circular RNAs (circRNAs) have been reported to have critical regulatory roles in tumor biology. However, their contribution to melanoma remains largely unknown. Methods CircRNAs derived from oncogene CD151 were detected and verified by analyzing a large number of melanoma samples through quantitative real-time polymerase chain reaction (qRT-PCR). Melanoma cells were stably transfected with lentiviruses using circ_0020710 interference or overexpression plasmid, and then CCK-8, colony formation, wound healing, transwell invasion assays, and mouse xenograft models were employed to assess the potential role of circ_0020710. RNA immunoprecipitation, luciferase reporter assay and fluorescence in situ hybridization were used to evaluate the underlying mechanism of circ_0020710. Results Our findings indicated that circ_0020710 was generally overexpressed in melanoma tissues, and high level of circ_0020710 was positively correlated with malignant phenotype and poor prognosis of melanoma patients. Elevated circ_0020710 promoted melanoma cell proliferation, migration and invasion in vitro as well as tumor growth in vivo. Mechanistically, we found that high level of circ_0020710 could upregulate the CXCL12 expression via sponging miR-370-3p. CXCL12 downregulation could reverse the malignant behavior of melanoma cells conferred by circ_0020710 over expression. Moreover, we also found that elevated circ_0020710 was correlated with cytotoxic lymphocyte exhaustion, and a combination of AMD3100 (the CXCL12 Conclusions Elevated circ_0020710 drives tumor progression via the miR-370-3p |
| 语种 | 英语 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/124243]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 作者单位 | 1.Hefei Institutes of Physical Science, Chinese Academy of Sciences 2.复旦大学 |
| 推荐引用方式 GB/T 7714 | Chuan-Yuan Wei,Meng-Xuan Zhu,Nan-Hang Lu,et al. Circular RNA circ_0020710 drives tumor progression and immune evasion by regulating the miR-370-3p[J]. Molecular Cancer,2020,19. |
| APA | Chuan-Yuan Wei.,Meng-Xuan Zhu.,Nan-Hang Lu.,Jia-Qi Liu.,Yan-Wen Yang.,...&Jian-Ying Gu.(2020).Circular RNA circ_0020710 drives tumor progression and immune evasion by regulating the miR-370-3p.Molecular Cancer,19. |
| MLA | Chuan-Yuan Wei,et al."Circular RNA circ_0020710 drives tumor progression and immune evasion by regulating the miR-370-3p".Molecular Cancer 19(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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