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Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy

文献类型：期刊论文

作者Lu, Pengpeng3,4; Zhang, Caiyun4; Fu, Lili3,4; Wei, Yinghui2,3; Huang, Yan3,4; Wang, Xiaoyan3,4; Lv, Changjun2; Chen, Lingxin1,3,4
刊名ANALYTICAL CHEMISTRY
出版日期2021-07-27
卷号93期号:29页码:10378-10387
ISSN号0003-2700
DOI10.1021/acs.analchem.1c02386
通讯作者Huang, Yan(yhuang@yic.ac.cn) ; Wang, Xiaoyan(wangxy@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Pantetheinase (also known as Vanin-1) is highly expressed in the liver, kidneys, and intestine and is closely associated with a number of diseases. Vanin-1 can hydrolyze pantetheine to pantothenic acid (vitamin B5) and cysteamine and participate in the synthesis of glutathione (GSH). GSH is highly expressed in tumor cells and plays a major role in the resistance of tumor cells to cisplatin. Therefore, we urgently need a method to monitor the activity level of Vanin-1 in tumor cells and tissues and elucidate the relationship between the role of Vanin-1 in GSH synthesis and tumor resistance. Herein, we report a Cy-Pa fluorescent probe for imaging Vanin-1 in cells and in vivo that can qualitatively and quantitatively detect the fluctuation of Vanin-1 concentrations in HepG2 and HepG2/DDP cells or tumor tissues of tumor-bearing mice. This probe shows excellent potential in in situ real-time monitoring of endogenous Vanin-1. Moreover, we proved that Vanin-1 can inhibit GSH synthesis using the probe. When the Vanin-1 inhibitor RR6 was used in combination with cisplatin, HepG2 and HepG2/DDP cells showed increased resistance to cisplatin, while the therapeutic efficiency of cisplatin was reduced in HepG2 and HepG2/DDP xenografts. In this study, Vanin-1 was shown to play an important role in the treatment of cancer, and the study of Vanin-1 may provide an idea for the treatment of cancer in the future.
WOS关键词DRUG-RESISTANCE ; URINARY VANIN-1 ; CANCER ; GLUTATHIONE ; CISPLATIN ; PANTETHEINASE ; ASSAY ; CYSTEAMINE ; METABOLISM ; PATHWAY
资助项目National Nature Science Foundation of China[81573393] ; National Nature Science Foundation of China[21804010] ; National Nature Science Foundation of China[21976209] ; National Nature Science Foundation of China[22007005] ; Science and Technology Innovation Development Plan of Yantai of China[2020MSGY113] ; Science and Technology Innovation Development Plan of Yantai of China[2020YT06000898] ; Research Initiation Fund of Binzhou Medical University[BY2019KYQD39] ; Research Initiation Fund of Binzhou Medical University[BY2020KYQD01] ; Taishan Scholar Project Special Fund[ts20190962] ; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization[2018LS014]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000679366000052
资助机构National Nature Science Foundation of China ; Science and Technology Innovation Development Plan of Yantai of China ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Fund ; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization
源URL[http://ir.yic.ac.cn/handle/133337/29551]  
专题烟台海岸带研究所_山东省海岸带环境工程技术研究中心
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Huang, Yan; Wang, Xiaoyan; Chen, Lingxin
作者单位1.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
2.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Lu, Pengpeng,Zhang, Caiyun,Fu, Lili,et al. Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy[J]. ANALYTICAL CHEMISTRY,2021,93(29):10378-10387.
APA Lu, Pengpeng.,Zhang, Caiyun.,Fu, Lili.,Wei, Yinghui.,Huang, Yan.,...&Chen, Lingxin.(2021).Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy.ANALYTICAL CHEMISTRY,93(29),10378-10387.
MLA Lu, Pengpeng,et al."Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy".ANALYTICAL CHEMISTRY 93.29(2021):10378-10387.

入库方式： OAI收割

来源：烟台海岸带研究所

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