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Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum

文献类型：期刊论文

作者Han, Yijing3,4,5; Zhang, Qianqian4,5; Chen, Lizhu2; Yang, Dinglong4,5; Zhao, Jianmin1,4,5
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2021-08-01
卷号121页码:10
关键词Ruditapes philippinarum Extracellular traps Reactive oxygen species Mitochondria Antibacterial activity
ISSN号0145-305X
DOI10.1016/j.dci.2021.104094
通讯作者Yang, Dinglong(dlyang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn)
英文摘要The formation of extracellular traps (ETs) is an important innate immune mechanism that serves to combat different invading pathogens. In this study, zymosan significantly induced the formation of ETs in the hemocytes of Ruditapes philippinarum, and this effect was accompanied by translocation of the mitochondria to the cell surface. Zymosan stimulation clearly induced an increase in intracellular ROS and MPO production and an overexpression of ROS-related genes (PI3K, AKT and HIF). In response to the ROS burst, the mitochondrial membrane potential decreased, and the mitochondrial permeability transition pore opened. Conversely, mitochondrial superoxide inhibitor (Mito-TEMPO) significantly inhibited the formation of ETs, suggesting that mitochondrial ROS were necessary for the formation of ETs. In addition, we found that zymosan-induced ETs showed antibacterial activities against gram-negative and gram-positive bacteria, such as Vibrio anguillarum, Vibrio harveyi, Escherichia coli and Micrococcus luteus. Taken together, these findings elucidated a new antibacterial approach for R. philippinarum and highlighted the role of mitochondria in the formation of zymosaninduced ETs.
WOS关键词VIBRIO-TAPETIS ; MANILA CLAMS ; DNA ; RELEASE ; MYELOPEROXIDASE ; IDENTIFICATION ; BACTERIA ; CARP
资助项目National Natural Science Foundation of China[41806196] ; Natural Science Foundation of Shandong Province[ZR2019BD022] ; Yantai Science and Technology Development Project[2020MSGY066] ; Youth Innovation Promotion Association, Chinese Academy of Sciences[2019216]
WOS研究方向Fisheries ; Immunology ; Veterinary Sciences ; Zoology
语种英语
WOS记录号WOS:000663315400003
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Association, Chinese Academy of Sciences
源URL[http://ir.yic.ac.cn/handle/133337/29433]  
专题中国科学院牟平海岸带环境综合试验站
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
海岸带生物资源高效利用研究与发展中心
通讯作者Yang, Dinglong; Zhao, Jianmin
作者单位1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
2.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China
5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Han, Yijing,Zhang, Qianqian,Chen, Lizhu,et al. Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,121:10.
APA Han, Yijing,Zhang, Qianqian,Chen, Lizhu,Yang, Dinglong,&Zhao, Jianmin.(2021).Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,121,10.
MLA Han, Yijing,et al."Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 121(2021):10.

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来源：烟台海岸带研究所

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