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Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses

文献类型：期刊论文

作者Han, Yijing3,4,5; Hu, Gege5; Chen, Yuying2; Chen, Lizhu2; Yu, Daode1; Zhang, Qianqian4,5; Yang, Dinglong4,5
刊名FRONTIERS IN MARINE SCIENCE
出版日期2021-07-16
卷号8页码:10
关键词Ruditapes philippinarum defensin extracellular traps antimicrobial effect membrane permeabilization
DOI10.3389/fmars.2021.690879
通讯作者Yu, Daode(yudaode@shandong.cn) ; Yang, Dinglong(dlyang@yic.ac.cn)
英文摘要Although defensins have been isolated from a variety of metazoan, their role in cellular immunity has not been answered. In the study, we found that the hemocytes of the Manila clams Ruditapes philippinarum release defensin (designated as Rpdef3) in response to Vibrio parahaemolyticus challenge. The antimicrobial Rpdef3 was proved to be involved in the extracellular traps (ETs) that hemocytes released in response to Vibrio challenge. Scanning electron microscopy observation proved the patterns how ETs eliminate invading bacteria. Furthermore, Rpdef3 involved in ETs had broad-spectrum antimicrobial effect on both Gram-negative bacteria and Gram-positive bacteria. ELISA assay revealed that Rpdef3 could bind lipopolysaccharides and peptidoglycan in a dose-dependent manner. As concerned to the antibacterial mechanisms, Rpdef3 can cause bacterial membrane permeabilization, leading to cell death. As a result, Rpdef3 might contribute to the trap and the elimination of invading Vibrio in clam ETs. Taken together, our study suggest that the formation of ETs is a defense mechanism triggered by bacterial stimulation, coupled with antibacterial defensin.
WOS关键词NEUTROPHIL EXTRACELLULAR TRAPS ; ESCHERICHIA-COLI ; ANTIBACTERIAL ACTIVITIES ; MEMBRANE ; PEPTIDES ; SHRIMP ; MECHANISMS ; HEMOCYTES ; JAPONICUS ; MOTILITY
资助项目National Natural Science Foundation of China[41806196] ; Natural Science Foundation of Shandong Province[ZR2019BD022] ; Yantai Science and Technology Development Project[2020MSGY066] ; Youth Innovation Promotion Association of CAS[2019216]
WOS研究方向Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种英语
WOS记录号WOS:000680690300001
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Association of CAS
源URL[http://ir.yic.ac.cn/handle/133337/29527]  
专题中国科学院牟平海岸带环境综合试验站
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
海岸带生物资源高效利用研究与发展中心
通讯作者Yu, Daode; Yang, Dinglong
作者单位1.Marine Sci Res Inst Shandong Prov, Qingdao, Peoples R China
2.Shandong Marine Resource & Environm Res Inst, Yantai, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai, Peoples R China
推荐引用方式
GB/T 7714		 Han, Yijing,Hu, Gege,Chen, Yuying,et al. Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses[J]. FRONTIERS IN MARINE SCIENCE,2021,8:10.
APA Han, Yijing.,Hu, Gege.,Chen, Yuying.,Chen, Lizhu.,Yu, Daode.,...&Yang, Dinglong.(2021).Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses.FRONTIERS IN MARINE SCIENCE,8,10.
MLA Han, Yijing,et al."Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses".FRONTIERS IN MARINE SCIENCE 8(2021):10.

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来源：烟台海岸带研究所

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