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Bacteriophytochrome from Magnetospirillum magneticum affects phototactic behavior in response to light

文献类型：期刊论文

作者Chen, Haitao3,4,5; Li, Dandan1; Cai, Yao7; Wu, Long-Fei2,6; Song, Tao3,4,5
刊名FEMS MICROBIOLOGY LETTERS
出版日期2020-09-01
卷号367期号:17页码:10
关键词bacteriophytochrome magnetotactic bacteria biliverdin phototactic behavior
ISSN号0378-1097
DOI10.1093/femsle/fnaa142
英文摘要Phytochromes are a class of photoreceptors found in plants and in some fungi, cyanobacteria, and photoautotrophic and heterotrophic bacteria. Although phytochromes have been structurally characterized in some bacteria, their biological and ecological roles in magnetotactic bacteria remain unexplored. Here, we describe the biochemical characterization of recombinant bacteriophytochrome (BphP) from magnetotactic bacteria Magnetospirillum magneticum AMB-1 (MmBphP). The recombinant MmBphP displays all the characteristic features, including the property of binding to biliverdin (BV), of a genuine phytochrome. Site-directed mutagenesis identified that cysteine-14 is important for chromophore covalent binding and photoreversibility. Arginine-240 and histidine-246 play key roles in binding to BV. The N-terminal photosensory core domain of MmBphP lacking the C-terminus found in other phytochromes is sufficient to exhibit the characteristic red/far-red-light-induced fast photoreversibility of phytochromes. Moreover, our results showed MmBphP is involved in the phototactic response, suggesting its conservative role as a stress protectant. This finding provided us a better understanding of the physiological function of this group of photoreceptors and photoresponse of magnetotactic bacteria.
WOS关键词MULTICELLULAR MAGNETOTACTIC PROKARYOTES ; CHROMOPHORE-BINDING DOMAIN ; HISTIDINE KINASES ; CRYSTAL-STRUCTURE ; PHYTOCHROME ; BACTERIA ; MODULATE ; REVEALS ; PROTEIN ; COMPLEX
资助项目National Natural Science Foundation of China[51937011] ; National Youth Foundation of China[51907192] ; Institute of Electrical Engineering, Chinese Academy of Sciences[Y650141CSA] ; CNRS-CAS
WOS研究方向Microbiology
语种英语
WOS记录号WOS:000602578400005
出版者OXFORD UNIV PRESS
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Youth Foundation of China ; National Youth Foundation of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences ; CNRS-CAS ; CNRS-CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Youth Foundation of China ; National Youth Foundation of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences ; CNRS-CAS ; CNRS-CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Youth Foundation of China ; National Youth Foundation of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences ; CNRS-CAS ; CNRS-CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Youth Foundation of China ; National Youth Foundation of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences ; CNRS-CAS ; CNRS-CAS
源URL[http://ir.iggcas.ac.cn/handle/132A11/99914]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Wu, Long-Fei; Song, Tao
作者单位1.Natl Inst Biol Sci, Beijing 102206, Peoples R China
2.Aix Marseille Univ, CNRS, LCB, F-13402 Marseille, France
3.Chinese Acad Sci, Inst Elect Engn, Beijing Key Lab Biol Electromagnetism, Beijing 100190, Peoples R China
4.Univ Chinese Acad Sci, Sch Elect Elect & Commun Engn, Beijing 100049, Peoples R China
5.Chinese Acad Sci, France China Int Lab Evolut & Dev Magnetotact Mul, Beijing 100190, Peoples R China
6.CNRS, France China Int Lab Evolut & Dev Magnetotact Mul, F-13402 Marseille, France
7.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Haitao,Li, Dandan,Cai, Yao,et al. Bacteriophytochrome from Magnetospirillum magneticum affects phototactic behavior in response to light[J]. FEMS MICROBIOLOGY LETTERS,2020,367(17):10.
APA Chen, Haitao,Li, Dandan,Cai, Yao,Wu, Long-Fei,&Song, Tao.(2020).Bacteriophytochrome from Magnetospirillum magneticum affects phototactic behavior in response to light.FEMS MICROBIOLOGY LETTERS,367(17),10.
MLA Chen, Haitao,et al."Bacteriophytochrome from Magnetospirillum magneticum affects phototactic behavior in response to light".FEMS MICROBIOLOGY LETTERS 367.17(2020):10.

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来源：地质与地球物理研究所

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