Intrathecal administration of the extracellular vesicles derived from human Wharton's jelly stem cells inhibit inflammation and attenuate the activity of inflammasome complexes after spinal cord injury in rats
文献类型：期刊论文
| 作者 | Noori, Leila7; Arabzadeh, Somayeh6; Mohamadi, Yousef5; Mojaverrostami, Sina7; Mokhtari, Tahmineh3,4 ; Akbari, Mohammad7; Hassanzadeh, Gholamreza1,2,7
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| 刊名 | NEUROSCIENCE RESEARCH
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| 出版日期 | 2021-09-01 |
| 卷号 | 170页码:87-98 |
| ISSN号 | 0168-0102 |
| 关键词 | Spinal cord injury Inflammasome Wharton's jelly mesenchymal stem cells Extracellular vesicles Intrathecal injection |
| DOI | 10.1016/j.neures.2020.07.011 |
| 通讯作者 | Hassanzadeh, Gholamreza(hassanzadeh@tums.ac.ir) |
| 英文摘要 | Activation of inflammasome complexes during spinal cord injury (SCI) lead to conversion of pro inflammatory cytokines, interleukin-1beta (IL-1 beta) and interleukin-18 (IL-18) to their active form to initiates the neuroinflammation. Mesenchymal stem cells (MSCs) showed anti-inflammatory properties through their extracellular vehicles (EVs). We investigated immunomodulatory potential of human Wharton's jelly mesenchymal stem cells derived extracellular vesicles (hWJ-MSC-EVs) on inflammasome activity one week after SCI in rats. The gene expression and protein level of IL-113, IL-18, tumor necrosis factor alpha (TNF-a) and caspase1, were assessed by QPCR and western blotting. Immunohistochemistry (IHC) was done to measure the glial fibrillary acidic protein (GFAP) and Nestin expression. Cell death, histological evaluation and hind limb locomotion was studied by TUNEL assay, Nissl staining and Basso, Beattie, Bresnaham (BBB), respectively. Our finding represented that intrathecally administrated of hWJMSC-EVs significantly attenuated expression of the examined factors in both mRNA (P < 0.05 and P < 0.01) and protein levels (P < 0.05 and P < 0.01), decreased GFAP and increased Nestin expression (P < 0.05), reduced cell death and revealed the higher number of typical neurons in ventral horn of spinal cord. Consequently, progress in locomotion. We came to the conclusion that hWJ-MSC-EVs has the potential to control the inflammasome activity after SCI in rats. Moreover, EVs stimulated the neural progenitor cells and modulate the astrocyte activity. (c) 2020 Elsevier B.V. and Japan Neuroscience Society. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | MESENCHYMAL STROMAL CELLS ; NEURAL PROGENITOR CELLS ; MOLECULAR PLATFORM ; THERAPEUTIC TARGET ; PROLIFERATION ; ACTIVATION ; EXOSOMES ; PROTECT ; MICROENVIRONMENT ; DIFFERENTIATION |
| 资助项目 | Tehran University of Medical Sciences[97013036613] |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| 出版者 | ELSEVIER IRELAND LTD |
| WOS记录号 | WOS:000680521000012 |
| 资助机构 | Tehran University of Medical Sciences |
| 源URL | [http://ir.psych.ac.cn/handle/311026/40071]  |
| 专题 | 心理研究所_中国科学院心理健康重点实验室 |
| 通讯作者 | Hassanzadeh, Gholamreza |
| 作者单位 | 1.Legal Med Org, Legal Med Res Ctr, Tehran, Iran 2.Univ Tehran Med Sci, Sch Adv Technol Med, Dept Neurosci & Addict Studies, Tehran, Iran 3.Univ Chinese Acad Sci, Dept Psychol, Beijing, Peoples R China 4.Inst Psychol, CAS Key Lab Mental Hlth, Beijing, Peoples R China 5.Ilam Univ Med Sci, Sch Med, Dept Anat, Ilam, Iran 6.Ale Taha Inst Higher Educ, Sch Basic Sci, Dept Biol, Tehran, Iran 7.Univ Tehran Med Sci, Sch Med, Dept Anat, Tehran, Iran |
| 推荐引用方式 GB/T 7714 | Noori, Leila,Arabzadeh, Somayeh,Mohamadi, Yousef,et al. Intrathecal administration of the extracellular vesicles derived from human Wharton's jelly stem cells inhibit inflammation and attenuate the activity of inflammasome complexes after spinal cord injury in rats[J]. NEUROSCIENCE RESEARCH,2021,170:87-98. |
| APA | Noori, Leila.,Arabzadeh, Somayeh.,Mohamadi, Yousef.,Mojaverrostami, Sina.,Mokhtari, Tahmineh.,...&Hassanzadeh, Gholamreza.(2021).Intrathecal administration of the extracellular vesicles derived from human Wharton's jelly stem cells inhibit inflammation and attenuate the activity of inflammasome complexes after spinal cord injury in rats.NEUROSCIENCE RESEARCH,170,87-98. |
| MLA | Noori, Leila,et al."Intrathecal administration of the extracellular vesicles derived from human Wharton's jelly stem cells inhibit inflammation and attenuate the activity of inflammasome complexes after spinal cord injury in rats".NEUROSCIENCE RESEARCH 170(2021):87-98. |
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来源：心理研究所
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