A DNA tetrahedral structure-mediated ultrasensitive fluorescent microarray platform for nucleic acid test
文献类型：期刊论文
| 作者 | Zhang, H; Liu, XX; Zhang, CX; Xu, Y; Su, J; Lu, XL; Shi, JY; Wang, LH; Landry, MP; Zhu, Y |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2020 |
| 卷号 | 321页码:- |
| ISSN号 | 0925-4005 |
| 关键词 | SIGNAL AMPLIFICATION MICRORNA DETECTION BIOSENSING INTERFACE SENSITIVE DETECTION NANOSTRUCTURES CANCER SENSOR DELIVERY ORIGAMI CARRIER |
| DOI | 10.1016/j.snb.2020.128538 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Microarrays are key platforms for biomolecule detection owing to their parallelizability and amenability for high-throughput. However, conventional fluorescent microarrays still suffer from low specificity and sensitivity, therefore are unsuitable for detection of low abundance nucleic acids. In this study, we demonstrate a universal microarray platform with high specificity and ultra-sensitivity for fluorescent detection of DNA and micro RNA (miRNA), which employs a DNA tetrahedral structured probe (DTSP) together with a hybridization chain reaction (HCR) based signal amplification technique. By precisely modulating the number of base pairs on each tetrahedron side, we developed three different sized DTSPs: 17 base pairs, 26 base pairs, and 37 base pairs (DTSP-17, DTSP-26 and DTSP-37, respectively). A low detection limit of 10 aM was obtained by DTSP-26, notably much lower than detection limits of prior methods. Furthermore, our microarray platform can distinguish single base DNA mismatches and thus exhibits single nucleotide specificity. Lastly, our microarray platform can be implemented for miRNA detection, as we demonstrate in a mimic medium, demonstrating potential for its use in clinical diagnosis. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32674]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Hangzhou Inst Adv Study, Key Lab Syst Biol, Hangzhou 310024, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China 3.Univ Calif Berkeley, Dept Chem & Biomol Engn, Berkeley, CA 94720 USA 4.CAS Ctr Excellence Superconducting Elect, CENSE, Shanghai 200050, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Key Lab Funct Mat Informat, Shanghai 200050, Peoples R China 6.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 8.Shanghai Biotechnol Corp, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, H,Liu, XX,Zhang, CX,et al. A DNA tetrahedral structure-mediated ultrasensitive fluorescent microarray platform for nucleic acid test[J]. SENSORS AND ACTUATORS B-CHEMICAL,2020,321:-. |
| APA | Zhang, H.,Liu, XX.,Zhang, CX.,Xu, Y.,Su, J.,...&Mi, XQ.(2020).A DNA tetrahedral structure-mediated ultrasensitive fluorescent microarray platform for nucleic acid test.SENSORS AND ACTUATORS B-CHEMICAL,321,-. |
| MLA | Zhang, H,et al."A DNA tetrahedral structure-mediated ultrasensitive fluorescent microarray platform for nucleic acid test".SENSORS AND ACTUATORS B-CHEMICAL 321(2020):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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