Axial resolution and contrast enhancement in digital scanned light-sheet microscopy via stimulated emission depletion
文献类型：期刊论文
| 作者 | Deng, SH; Li, XH; Ding, ZJ; Zhang, YL; Liu, MP; Zhou, HL; Wang, YH |
| 刊名 | JOURNAL OF OPTICS
 |
| 出版日期 | 2020 |
| 卷号 | 22期号:10页码:- |
| 关键词 | SELF-RECONSTRUCTING BEAMS ADAPTIVE OPTICS ILLUMINATION MICROSCOPY FLUORESCENCE MICROSCOPY STED MICROSCOPY DEEP DIFFRACTION ABERRATIONS FIELD SPIM |
| ISSN号 | 2040-8978 |
| DOI | 10.1088/2040-8986/abb1d3 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Integration of stimulated emission depletion (STED) with digital scanned light-sheet microscopy (DSLM) using a Bessel beam achieves fast, high-resolution and large field of view (FOV) imaging. Here, we report on a combination method of DSLM and STED by using a Bessel beam for excitation and a hollow Gaussian beam for depletion. Owing to the advantages of confined axial distribution around the focal plane for the hollow Gaussian depletion beam, those fluorescence signals from the periphery of the Bessel excitation pattern are depleted by stimulated emission. A super-resolved light sheet with suppressed side lobes is achieved. The numerical simulation results show that the use of the Bessel beam for the excitation and the hollow Gaussian beam for the STED results in a DSLM system with enhanced performance of the axial resolution and image contrast over a moderately large FOV. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32721]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China 2.Nanchang Univ, Sch Informat Engn, Nanchang 330031, Jiangxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, SH,Li, XH,Ding, ZJ,et al. Axial resolution and contrast enhancement in digital scanned light-sheet microscopy via stimulated emission depletion[J]. JOURNAL OF OPTICS,2020,22(10):-. |
| APA | Deng, SH.,Li, XH.,Ding, ZJ.,Zhang, YL.,Liu, MP.,...&Wang, YH.(2020).Axial resolution and contrast enhancement in digital scanned light-sheet microscopy via stimulated emission depletion.JOURNAL OF OPTICS,22(10),-. |
| MLA | Deng, SH,et al."Axial resolution and contrast enhancement in digital scanned light-sheet microscopy via stimulated emission depletion".JOURNAL OF OPTICS 22.10(2020):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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