Cytoskeleton induced the changes of microvilli and mechanical properties in living cells by atomic force microscopy
文献类型：期刊论文
| 作者 | Liu, XY; Wei, YH; Li, W; Li, B; Liu, L |
| 刊名 | JOURNAL OF CELLULAR PHYSIOLOGY
 |
| 页码 | - |
| 关键词 | ACTIN BUNDLES MEMBRANE MICROTUBULES ELASTICITY ACTIVATION TOOL |
| ISSN号 | 0021-9541 |
| DOI | 10.1002/jcp.30110 |
| 文献子类 | 期刊论文 |
| 英文摘要 | The cytoskeleton acts as a scaffold for membrane protrusion, such as microvilli. However, the relationship between the characteristics of microvilli and cytoskeleton remains poorly understood under the physiological state. To investigate the role of the cytoskeleton in regulating microvilli and cellular mechanical properties, atomic force microscopy (AFM) was used to detect the dynamic characteristics of microvillus morphology and elastic modulus of living HeLa cells. First, HeLa and MCF-7 cell lines were stained with Fluor-488-phalloidin and microtubules antibody. Then, the microvilli morphology was analyzed by high-resolution images of AFM in situ. Furthermore, changes in elastic modulus were investigated by the force curve of AFM. Fluorescence microscopy and AFM results revealed that destroyed microfilaments led to a smaller microvilli size, whereas the increase in the aggregation and number of microfilaments led to a larger microvilli size. The destruction and aggregation of microfilaments remarkably affected the mechanical properties of HeLa cells. Microtubule-related drugs induced the change of microtubule, but we failed to note significant differences in microvilli morphology and mechanical properties of cells. In summary, our results unraveled the relationship between microfilaments and the structure of microvilli and Young's modulus in living HeLa cells, which would contribute to the further understanding of the physiological function of the cytoskeleton in vivo. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32775]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, CAS Key Lab Interfacial Phys & Technol, Div Phys Biol,Shanghai Inst Appl Phys, Shanghai, Peoples R China 2.Wenzhou Med Univ, Minist Educ China, Sch Lab Med & Life Sci, Key Lab Med, Wenzhou 325035, Peoples R China 3.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, CAS Key Lab Interfacial Phys & Technol, Bioimaging Ctr,Shanghai Inst Appl Phys, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China 5.Wenzhou Med Univ, Zhejiang Prov Key Lab Med Genet, Wenzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, XY,Wei, YH,Li, W,et al. Cytoskeleton induced the changes of microvilli and mechanical properties in living cells by atomic force microscopy[J]. JOURNAL OF CELLULAR PHYSIOLOGY:-. |
| APA | Liu, XY,Wei, YH,Li, W,Li, B,&Liu, L. |
| MLA | Liu, XY,et al."Cytoskeleton induced the changes of microvilli and mechanical properties in living cells by atomic force microscopy".JOURNAL OF CELLULAR PHYSIOLOGY |
入库方式： OAI收割
来源：上海应用物理研究所
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