Exosomes mediate an epithelial-mesenchymal transition cascade in retinal pigment epithelial cells: Implications for proliferative vitreoretinopathy
文献类型：期刊论文
| 作者 | Zhang, Y; Wang, KZ; Pan, JB; Yang, S; Yao, HP; Li, M; Li, H; Lei, HT; Jin, HY; Wang, F |
| 刊名 | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
 |
| 出版日期 | 2020 |
| 卷号 | 24期号:22页码:13324-13335 |
| 关键词 | EXTRACELLULAR VESICLES POLYETHYLENE-GLYCOL RISK-FACTORS CANCER PROMOTES MICRORNAS CARCINOMA INVASION RNA MICROVESICLES |
| ISSN号 | 1582-1838 |
| DOI | 10.1111/jcmm.15951 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Exosomes have recently emerged as a pivotal mediator of many physiological and pathological processes. However, the role of exosomes in proliferative vitreoretinopathy (PVR) has not been reported. In this study, we aimed to investigate the role of exosomes in PVR. Transforming growth factor beta 2 (TGFss-2) was used to induce epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells, as an in vitro model of PVR. Exosomes from normal and EMTed RPE cells were extracted and identified. We incubated extracted exosomes with recipient RPE cells, and co-cultured EMTed RPE cells and recipient RPE cells in the presence of the exosome inhibitor GW4869. Both experiments suggested that there are further EMT-promoting effects of exosomes from EMTed RPE cells. MicroRNA sequencing was also performed to identify the miRNA profiles in exosomes from both groups. We identified 34 differentially expressed exosomal miRNAs (P <. 05). Importantly, miR-543 was found in exosomes from EMTed RPE cells, and miR-543-enriched exosomes significantly induced the EMT of recipient RPE cells. Our study demonstrates that exosomal miRNA is differentially expressed in RPE cells during EMT and that these exosomal miRNAs may play pivotal roles in EMT induction. Our results highlight the importance of exosomes as cellular communicators within the microenvironment of PVR. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32868]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Ctr Translat Neurodegenerat & Regenerat Therapy, Shanghai, Peoples R China 2.Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Ophthalmol, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Bioimaging Ctr, Shanghai, Peoples R China 5.Harvard Med Sch, Schepens Eye Res Inst Massachusetts Eye & Ear, Dept Ophthalmol, Boston, MA 02115 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Y,Wang, KZ,Pan, JB,et al. Exosomes mediate an epithelial-mesenchymal transition cascade in retinal pigment epithelial cells: Implications for proliferative vitreoretinopathy[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2020,24(22):13324-13335. |
| APA | Zhang, Y.,Wang, KZ.,Pan, JB.,Yang, S.,Yao, HP.,...&Wang, F.(2020).Exosomes mediate an epithelial-mesenchymal transition cascade in retinal pigment epithelial cells: Implications for proliferative vitreoretinopathy.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,24(22),13324-13335. |
| MLA | Zhang, Y,et al."Exosomes mediate an epithelial-mesenchymal transition cascade in retinal pigment epithelial cells: Implications for proliferative vitreoretinopathy".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 24.22(2020):13324-13335. |
入库方式： OAI收割
来源：上海应用物理研究所
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