Hydrogen molecules can modulate enzymatic activity and structural properties of pepsin in vitro
文献类型：期刊论文
| 作者 | Cheng, J; Tang, C; Li, XL; Hu, J; Lu, J |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2020 |
| 卷号 | 189页码:- |
| 关键词 | MEDIATES SUPPRESSION COLON INFLAMMATION NANOPARTICLES EXPRESSION RELEASE GAS |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2020.110856 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Hydrogen gas therapy has been recognized as the promising application merit. However, the underlying mechanism on the biological effects remains far from being understood. In this work, pepsin used as a research model, the effects of hydrogen-rich water on the protein activities and structural properties were investigated by enzymatic assay, atomic force microscopy-based peakforce quantitative nanomechanical mapping (PF-QNM) and terahertz time-domain spectroscopy (THz-TDS). We found that hydrogen-rich water can increase the protein activity and its apparent height while alter the mechanical properties (Young's modulus) and the terahertz dynamics. These results suggest a possible mode of hydrogen molecules acting with pepsin through the local changes of hydrophobic interfaces in the protein molecules, thus provide the first evidence for the direct interaction between hydrogen with proteins and a biophysical insight into the mechanism of hydrogen as well as other gases on the biological effects. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32916]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shanghai Univ Med & Hlth Sci, Natl Engn Res Ctr Nanotechnol, Shanghai 201318, Peoples R China 2.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai Synchrotron Radiat Facil, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, J,Tang, C,Li, XL,et al. Hydrogen molecules can modulate enzymatic activity and structural properties of pepsin in vitro[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2020,189:-. |
| APA | Cheng, J,Tang, C,Li, XL,Hu, J,&Lu, J.(2020).Hydrogen molecules can modulate enzymatic activity and structural properties of pepsin in vitro.COLLOIDS AND SURFACES B-BIOINTERFACES,189,-. |
| MLA | Cheng, J,et al."Hydrogen molecules can modulate enzymatic activity and structural properties of pepsin in vitro".COLLOIDS AND SURFACES B-BIOINTERFACES 189(2020):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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