Impact of grazing on shaping abundance and composition of active methanotrophs and methane oxidation activity in a grassland soil
文献类型：期刊论文
| 作者 | Li, Y; Liu, YW; Pan, H; Hernandez, M; Guan, XM; Wang, W; Zhang, QC; Luo, Y; Di, HJ; Xu, JM |
| 刊名 | BIOLOGY AND FERTILITY OF SOILS
 |
| 出版日期 | 2020 |
| 卷号 | 56期号:6页码:799-810 |
| 关键词 | GREENHOUSE-GAS EMISSIONS NITROUS-OXIDE EMISSIONS DOMINATE NITRIFICATION OXIDIZING BACTERIA SEMIARID STEPPE INNER-MONGOLIA AMMONIA COMMUNITIES DIVERSITY BIOCHAR |
| ISSN号 | 0178-2762 |
| DOI | 10.1007/s00374-020-01461-0 |
| 文献子类 | 期刊论文 |
| 英文摘要 | The effect of grazing on the abundance, composition, and methane (CH4) uptake of methanotrophs in grasslands has been well documented in the past few decades, but the dominant communities of active methanotrophs responsible for CH4 oxidation activity in grazed soils are still poorly understood. In this study, we characterized the metabolically active, aerobic methanotrophs in grasslands with three different levels of grazing (light, medium, and heavy) by combining DNA-stable isotope probing (SIP) and quantitative PCR (qPCR) for methane monooxygenase (pmoA) gene- and 16S rRNA gene-based amplicon sequencing. The CH4 oxidation potential was as low as 0.51 mu mol g dry weight(-1) day(-1) in the ungrazed control, while it decreased as grazing intensity increased in grazed fields, ranging from 2.25 mu mol g dry weight(-1) day(-1) in light grazed fields to 1.59 in heavily grazed fields. Increased CH4 oxidation activity was paralleled by twofold increases in abundance of pmoA genes and relative abundance of methanotroph-affiliated 16S rRNA genes in the total microbial community in grazed soils. SIP and sequencing revealed that the genera Methylobacter and Methylosarcina (type I; Gammaproteobacteria) and Methylocystis (type II; Alphaproteobacteria) were active methanotrophs responsible for CH4 oxidation in grazed soils. Light and intermediate grazing stimulated the growth and activity of methanotrophs, while heavy grazing decreased the abundance and diversity of the active methanotrophs in the typical steppe. Redundancy and correlation analysis further indicated that the variation of bulk density and soil C and N induced by grazing determined the abundance, diversity of active methanotrophs, and methane oxidation activity in the long-term grazed grassland soil. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32920]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China 2.Zhejiang Univ, Zhejiang Prov Key Lab Agr Resources & Environm, Inst Soil & Water Resources & Environm Sci, Hangzhou 310058, Peoples R China 3.Shandong Agr Univ, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Coll Resources & Environm Sci, Tai An 271018, Shandong, Peoples R China 4.Univ Southampton, Ctr Biol Sci, Southampton SO17 1BJ, Hants, England |
| 推荐引用方式 GB/T 7714 | Li, Y,Liu, YW,Pan, H,et al. Impact of grazing on shaping abundance and composition of active methanotrophs and methane oxidation activity in a grassland soil[J]. BIOLOGY AND FERTILITY OF SOILS,2020,56(6):799-810. |
| APA | Li, Y.,Liu, YW.,Pan, H.,Hernandez, M.,Guan, XM.,...&Xu, JM.(2020).Impact of grazing on shaping abundance and composition of active methanotrophs and methane oxidation activity in a grassland soil.BIOLOGY AND FERTILITY OF SOILS,56(6),799-810. |
| MLA | Li, Y,et al."Impact of grazing on shaping abundance and composition of active methanotrophs and methane oxidation activity in a grassland soil".BIOLOGY AND FERTILITY OF SOILS 56.6(2020):799-810. |
入库方式： OAI收割
来源：上海应用物理研究所
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